Rhodamine B (Basic Violet 10) is a fluorescent dye used as a tracer dye in water to determine the rate and direction of flow and transport, with excitation/emission maxima at 568/583nm[1, 2]. Rhodamine B is commonly used in fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA in biotechnology experiments. It can be used to label organelles or cell membranes, and can also be coupled to antibodies to detect the presence and quantity of specific proteins[3, 4]. Rhodamine B is commonly used in industry for dyeing textiles, paper, soap, leather, and pharmaceuticals[5].
References:
[1] Field M S, Wilhelm R G, Quinlan J F, et al. An assessment of the potential adverse properties of fluorescent tracer dyes used for groundwater tracing[J]. Environmental Monitoring and Assessment, 1995, 38: 75-96.
[2] Stobiecka M, Hepel M. Multimodal coupling of optical transitions and plasmonic oscillations in rhodamine B modified gold nanoparticles[J]. Physical Chemistry Chemical Physics, 2011, 13(3): 1131-1139.
[3] Brulikova L, Krupkova S, Labora M, et al. Synthesis and study of novel pH-independent fluorescent mitochondrial labels based on Rhodamine B[J]. RSC advances, 2016, 6(28): 23242-23251.
[4] Ullman E F, Schwarzberg M, Rubenstein K E. Fluorescent excitation transfer immunoassay. A general method for determination of antigens[J]. Journal of Biological Chemistry, 1976, 251(14): 4172-4178.
[5] Farhan A M, Zaghair A M, Abdullah H I. Adsorption Study of Rhodamine–B Dye on Plant (Citrus Leaves)[J]. Baghdad Science Journal, 2022, 19(4): 0838-0838.
Rhodamine B (Basic Violet 10)是一种荧光染料，在水中用作示踪染料，以确定流动和运输的速率和方向，最大激发光/发射光为568/583nm[1, 2]。Rhodamine B在生物技术实验中常用于荧光显微镜、流式细胞术、荧光相关光谱和ELISA，可用于标记细胞器或细胞膜，还可以通过偶联到抗体上检测特定蛋白质的存在和数量[3, 4]。Rhodamine B在工业上常用于染色纺织品、纸张、肥皂、皮革和药品[5]。