Ranitidine hydrochloride

Cell lines

SVN neurons

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

1 μM

Applications

Ranitidine at 1 μM potently blocked the excitatory responses of SVN neurons to histamine (1 ~ 30 μM). Ranitidine significantly reduced the 23.6%, 35.1% and 48.7% increases in the peak firing rate induced by 1, 3 and 10 μM histamine to 7.1%, 13.7% and 18.3%, respectively. In addition, Ranitidine (1 μM) in combination with Mepyramine (1 μM) almost completely blocked the histamine-induced excitation.

Animal models

Wistar rats

Dosage form

25 mg/mL, 2 drops

Applications

In Wistar rats, Ranitidine induced progressive vasoconstriction, with a maximum of 27% decrease in the vascular diameter at 300th s. The administration of histamine after Ranitidine increased the vascular diameter which was relative to the value at 300th s. However, at 600th s, the vascular diameter still could not reach the initial value (at 0th s).

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Zhuang QX, Wu YH, Wu GY, Zhu JN, Wang JJ. Histamine excites rat superior vestibular nuclear neurons via postsynaptic H1 and H2 receptors in vitro. Neurosignals. 2013;21(3-4):174-83.

[2]. Coman OA, Rotar C, Stoleru S, Ghi-Cristescu I, Punescu H, Fulga I. Influencing vascular reactivity in vivo by histaminergic agonists and antagonists. Rom J Morphol Embryol. 2007;48(4):403-6.