Py-BODIPY-NHS ester is a cell-permeable and fluorescent energy transfer probe. Py-BODIPY-NHS ester tracer yields strong cellular engagement and proves to be optimal for MET kinase. The live-cell energy transfer technique can perform a broad-coverage, quantitative analysis of intracellular kinase target engagement[1][2].
Triphenylphosphonium-fluorochromes (TPP-fluorochromes) are a new class of spectrally variable, mitochondrially targeted probes. NanoBRET 590 SE (compound 2b) is synthesized by reacting the TPP-amine with NHS esters of BODIPY red[3].
[1]. Vasta JD, et al. Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of CellularATP on Target Engagement. Cell Chem Biol. 2018 Feb 15;25(2):206-214.e11. [2]. Robers MB, et al. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer. Methods Mol Biol. 2019;1888:45-71. [3]. Yuan H, et al. Fluorescent and radiolabeled triphenylphosphonium probes for imaging mitochondria. Chem Commun (Camb). 2013 Nov 14;49(88):10361-3.