1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) is an anionic phospholipid that plays a significant role in the structure and function of cell membranes[1]. 1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) is wildly used in the preparation of liposomes and other artificial membrane systems for studying the behavior of membrane proteins[2], drug delivery mechanisms[3][4], and the structure and function of cell membranes[5].
1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) can suppress the production of inflammatory mediators induced by recombinant S100A9 through both TLR2 and TLR4 pathways. In RAW264.7 cells, treatment with 100mg/ml DOPG and 2.5mg/ml S100A9 for 2 hours significantly inhibit the expression of IL-1β, IL-6, and TNF-α (tumor necrosis factor-α)[6].
1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) in water (prepared by sonicating a 1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) lipid film in deionized water) applied to the imiquimod (IMQ) treated mouse skin can inhibited IMQ-induced psoriasiform phenotype such as ear thickness and epidermal hyperplasia[6].
References:
[1]. Dinh BT, Price SE, Majul A, et al. Diazeniumdiolate reactivity in model membrane systems. Nitric Oxide. 2008 Mar;18(2):113-21. doi: 10.1016/j.niox.2007.11.001. Epub 2007 Nov 21. PMID: 18068133; PMCID: PMC2268087.
[2]. Balali-Mood K, Harroun TA, Bradshaw JP. Molecular dynamics simulations of a mixed DOPC/DOPG bilayer. Eur Phys J E Soft Matter. 2003 Nov;12 Suppl 1:S135-40. doi: 10.1140/epjed/e2003-01-031-3. Epub 2003 Nov 5. PMID: 15011033.
[3]. Srinivasan C, Burgess DJ. Optimization and characterization of anionic lipoplexes for gene delivery. J Control Release. 2009 May 21;136(1):62-70. doi: 10.1016/j.jconrel.2009.01.022. Epub 2009 Feb 5. PMID: 19331848.
[4]. Zhou S, Luo Y, Lovell JF. Vaccine approaches for antigen capture by liposomes. Expert Rev Vaccines. 2023 Jan-Dec;22(1):1022-1040. doi: 10.1080/14760584.2023.2274479. Epub 2023 Nov 6. PMID: 37878481; PMCID: PMC10872528.
[5]. Dargel C, Gräbitz-Bräuer F, Geisler R, et al. Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules. 2021 Aug 16;26(16):4959. doi: 10.3390/molecules26164959. PMID: 34443547; PMCID: PMC8399256.
[6]. Choudhary V, Uaratanawong R, Patel RR, et al. Phosphatidylglycerol Inhibits Toll-Like Receptor-Mediated Inflammation by Danger-Associated Molecular Patterns. J Invest Dermatol. 2019 Apr;139(4):868-877. doi: 10.1016/j.jid.2018.10.021. Epub 2018 Oct 31. PMID: 30391260; PMCID: PMC7309510.
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)是一种阴离子磷脂，在细胞膜的结构和功能中起着重要作用[1]。1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) 广泛用于制备脂质体和其他人工膜系统，以研究膜蛋白的行为[2]、药物传递机制[3] [4]以及细胞膜的结构和功能[5]。
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)可以通过TLR2和TLR4途径抑制重组S100A9蛋白引起的炎性介质的产生。在RAW264.7细胞中用100mg/ml的1,2-Dioleoyl-sn-glycero-3-PG (sodium salt)和2.5mg/ml的S100A9处理2小时可以显著抑制IL-1β、IL-6和TNF-α (肿瘤坏死因子-α) 的表达[6]。
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)溶液（通过在去离子水中超声处理1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)脂质膜制备）涂抹在咪喹莫特（IMQ）处理的小鼠皮肤上，可以抑制IMQ引起的银屑病样表型，如耳朵增厚和表皮增生[6]。