Pseudouridine-5'-triphosphate (Pseudo-UTP) is one of the most commonly used modified nucleoside for the polymerase-mediated synthesis of RNA molecules. Compared with uridine-containing unmodified mRNAs, the application of pseudouridine-containing modified mRNAs exhibits better nuclease stability, immunogenicity, and translational properties[1].
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[1]. Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62.