Prolactin releasing peptide (PrRP) is a 31-amino acid neuropeptide involved in regulating food intake. It is found in the hypothalamus, medulla, and pituitary in rats. PrRP is an agonist of the G-protein coupled receptors GPR10/hGR3 and neuropeptide FF receptor (hNPFF2; Kis = 1 and 19 nM, respectively). It stimulates calcium mobilization in HEK293 cells expressing GPR10 (EC50 = 1.5 nM) and radiolabeled GTPγS binding to membrane homogenates from CHO cells transfected with hNPFF2 (EC50 = 240 nM). PrRP (100 nM) increases the release of gonadotropin-releasing hormone , vasoactive intestinal peptide-, and galanin from rat medial basal hypothalamic explants. It increases plasma levels of luteinizing hormone, follicle stimulating hormone, and testosterone in male rats when administered at an intracerebroventricular dose of 5 nmol. PrRP reduces food intake in fasted rats to 58.4, 53.4, and 55.4% of control levels when administered at doses of 1, 5, and 10 nmol, respectively.
References:
[1]. Seal, L.G., Small, C.J., Shillo, W.S., et al. PRL-releasing peptide inhibits food intake in male rats via the dorsomedial hypothalamic nucleus and not the paraventricular hypothalamic nucleus Endocrinology 142(10), 4236-4243 (2001).
[2].Langmead, C.J., Szekeres, P.G., Chambers, J.K., et al. Characterization of the binding of [125I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor Br. J. Pharmacol. 131(4), 683-688 (2000).
[3]. Engstr?m, M., Brandt, A., Wurster, S., et al. Prolactin releasing peptide has high affinity and efficacy at neuropeptide FF2 receptors J. Pharmacol. Exp. Ther. 305(3), 825-832 (2003).
[4].Seal, L.J., Small, C.J., Kim, M.S., et al. Prolactin releasing peptide (PrRP) stimulates luteinizing hormone (LH) and follicle stimulating hormone (FSH) via a hypothalamic mechanism in male rats Endocrinology 141(5), 1909-1912 (2000).