PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1].
To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide.PIPES buffer can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].
[1]. Jason Moggridge, et al. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005.