Penicillin G Sodium是一种含有β-内酰胺环的抗生素，对敏感微生物有效。
Cas No.:69-57-8
Sample solution is provided at 25 µL, 10mM.
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Penicillin G Sodium is an antibiotic containing a β-lactam ring against sensitive organisms [1]. Penicillin G Sodium binds to penicillin-binding proteins (PBPs) of bacteria which are involved in cell wall synthesis and thus inhibit bacterial cell wall synthesis [2]. Penicillin G Sodium has been widely used to kill Staphylococcus aureus, and to develop a combined therapy with lactoferrin to induce the lysis of various bacteria and changes in the cell wall[3].
In vitro, Penicillin G Sodium treatment (150U/ml) for 48 hours led to the formation of abnormal Chlamydia trachomatis (CT) inclusions within HeLa cells infected with CT and without causing cytotoxicity, and altered the mRNA expression profile of CT[4].
In vivo, Penicillin G Sodium treatment (160mg/kg; s.c.) combined with gentamicin (10mg/kg; s.c.) twice daily for 5 days can significantly increase the survival rate of mice with penicillin-resistant pneumococcal pneumonia [5].
References:
[1] Chu L, Zhuang S, Wang J. Degradation kinetics and mechanism of penicillin G in aqueous matrices by ionizing radiation[J]. Radiation Physics and Chemistry, 2018, 145: 34-38.
[2] Li D, Yang M, Hu J, et al. Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river[J]. Water Research, 2008, 42(1-2): 307-317.
[3] Diarra M S, Lacasse P, Deschênes E, et al. Ultrastructural and cytochemical study of cell wall modification by lactoferrin, lactoferricin and penicillin G against Staphylococcus aureus[J]. Journal of electron microscopy, 2003, 52(2): 207-215.
[4] Wang M, Qiu X, Xu Y, et al. Impacts of penicillin G on Chlamydia trachomatis serovar D and host HeLa cells in genital infections[J]. Archives of Microbiology, 2025, 207(7): 164.
[5] Tateda K, Matsumoto T, Miyazaki S, et al. Efficacy of β-lactam antibiotics combined with gentamicin against penicillin-resistant pneumococcal pneumonia in CBA/J mice[J]. Journal of Antimicrobial Chemotherapy, 1999, 43(3): 367-371.
Penicillin G Sodium是一种含有β-内酰胺环的抗生素，对敏感微生物有效[1]。Penicillin G Sodium可与细菌中参与细胞壁合成的青霉素结合蛋白结合，从而抑制细菌细胞壁的合成[2]。Penicillin G Sodium已被广泛用于杀灭金黄色葡萄球菌，并与乳铁蛋白一起开发联合疗法，以诱导多种细菌的裂解和细胞壁的改变[3]。
在体外，150U/ml的Penicillin G Sodium处理感染沙眼衣原体(CT)的HeLa细胞48小时，导致异常CT包涵体的形成，且不产生细胞毒性，同时改变了CT的mRNA表达谱 [4]。
在体内，Penicillin G Sodium（160mg/kg；s.c.）联合庆大霉素（10 mg/kg；s.c.），每日两次，连续5天，可显著提高青霉素耐药肺炎球菌肺炎小鼠的存活率[5]。
| Cell experiment [1]: | |
Cell lines | HeLa cells |
Preparation Method | HeLa cell suspensions (5×104 cells/mL) were seeded into 6-well plates (2ml per well). Upon reaching a confluence of 80%, the culture medium was removed. Then the cells were treated with 30µg/ml of diethylaminoethyl dextran (DEAE-D) for 30min. Afterwards, the cells were cultured with serum-free Dulbecco’s modified Eagle’s medium (DMEM). The D/UW-3/CX strain of CT, containing an inclusion forming unit (IFU) concentration of 4.442×106 IFU/ml, was then inoculated onto the cells, followed by incubating at 37°C with 5% CO2 for 1h. The cells were then centrifuged at 500×g for 1h at 32°C, and the supernatant was removed. Fresh DMEM containing Penicillin G Sodium at a final concentration of 150U/ml was added to the wells and cultured for 48h. Cells with aberrant inclusions were selected using a microscope for further analysis. Cells were collected in 1.5ml tubes and centrifuged at 250×g for 10min at 32°C, fixed with glutaraldehyde, and subsequently examined using transmission electron microscopy (TEM). |
Reaction Conditions | 150U/ml; 48h |
Applications | Penicillin G Sodium treatment significantly induced formation of abnormal CT inclusions within HeLa cells. |
References: | |
| Cas No. | 69-57-8 | SDF | |
| 别名 | 青霉素G钠盐; 苄青霉素钠; Benzylpenicillin sodium salt | ||
| 化学名 | sodium;(2S,5R,6R)-3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate | ||
| Canonical SMILES | CC1(C(N2C(S1)C(C2=O)NC(=O)CC3=CC=CC=C3)C(=O)[O-])C.[Na+] | ||
| 分子式 | C16H18N2O4S.Na | 分子量 | 356.37 |
| 溶解度 | ≥ 13.7mg/mL in DMSO | 储存条件 | Store at -20°C,unstable in solution, ready to use. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8061 mL | 14.0304 mL | 28.0607 mL |
| 5 mM | 561.2 μL | 2.8061 mL | 5.6121 mL |
| 10 mM | 280.6 μL | 1.403 mL | 2.8061 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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- Purity: >98.00% Appearance: A solid
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