Fluorescent potent PARP inhibitor (IC50 = 12 nM). Composed of a PARP inhibitor conjugated to BDY FL, SE . Colocalizes with PARP immunostaining in multiple cancer cell lines in vivo and accumulates in tumor cells and tumor associated macrophages. Enables high temporal and subcellular spatial resolution of drug distribution. Emission max = 525 nm. Cell permeable.
Thurber et al (2013) Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo. Nat.Commun. 4 1504 PMID:23422672 |Thurber et al (2014) Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol.Cancer Ther. 13 986 PMID:24552776 |Dubach et al (2017) Quantitating drug-target engagement in single cells in vitro and in vivo. Nat.Chem.Biol. 13 168 PMID:27918558