Paradol

Cell experiment:

KB, human oral epidermoid carcinoma cell lines (ATCC CCL-17) are plated at a density of 5×103 cells/200 μL/well into 96-well plate. After an overnight growth, the cells are treated with a series of paradol derivatives. All of the derivatives of paradol tested are dissolved in DMSO. The final concentration of DMSO in the culture medium is kept below 0.1% and the controls are treated with DMSO alone. Cell viability is assessed using MTT assay. In brief, after the cells are grown in the media in the absence or presence of the test compounds (e.g., Paradol, 10, 50, 100, 150, and 200 μM) for 48 h, they are then replaced to a 200 μL culture medium containing 0.5 mg/mL MTT for 3 h. The resulting MTT-formazan product is dissolved by an addition of the same volume of DMSO. The amount of formazan is determined by measuring the absorbance at 570 nm[2].

Animal experiment:

Mice[3] Male ICR mice (7 weeks old, 36±2 g) challenged with middle cerebral artery occlusion (MCAO)/reperfusion (M/R) are randomly divided into vehicle (10% Tween80)- or Paradol-administered groups (n=6~7 per group). Paradol dissolved in 10% Tween80 is orally administered (10 mg/kg) into mice at 1, 5, or 10 mg/kg immediately after reperfusion. Rats[4] Five-week-old Sprague-Dawley rats (male) are used. At 8 weeks of age, the rats are fasted for 14 h prior to the oral administration of olive oil (1 mL) containing zingerone or 6-, 8-, or 12-paradol (10 mg/kg). Three rats in each group are anesthetized with isoflurane, and samples (0.3 mL) of their blood are collected from their jugular vein using a heparinized needle and syringe at 0 (i.e., prior to the oral administration), 0.25, 0.5, 1, 3, 6, and 24 h after the oral administration of the olive oil containing test compounds. The AUC0-24h values determined using this time schedule are very similar compared with AUC0-24h that sampled the time points more minutely with other materials in our laboratory.

References:

[1]. van Breemen RB, et al. Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale). Fitoterapia. 2011 Jan;82(1):38-43.
[2]. Keum YS, et al. Induction of apoptosis and caspase-3 activation by chemopreventive [6]-paradol and structurally related compounds in KB cells. Cancer Lett. 2002 Mar 8;177(1):41-7.
[3]. Gaire BP, et al. Neuroprotective effect of 6-paradol in focal cerebral ischemia involves the attenuation of neuroinflammatory responses in activated microglia. PLoS One. 2015 Mar 19;10(3):e0120203.
[4]. Setoguchi S, et al. Pharmacokinetics of Paradol Analogues Orally Administered to Rats. J Agric Food Chem. 2016 Mar 9;64(9):1932-7.