Oleoyl ethanolamide-d2 (OEA-d2) contains two deuterium atoms at the 11 position. It is intended for use as an internal standard for the quantification of OEA by GC- or LC-mass spectrometry. OEA is an analogue of the endocannabinoid arachidonoyl ethanolamide (AEA) found in brain tissue and in chocolate.1 It is one of the long chain fatty acid ethanolamides that accumulates rapidly in infarcted tissue,2 but its biosynthesis is reduced in the intestine of rats following food deprivation.3 OEA is an endogenous, potent agonist for peroxisome proliferator-activated receptor α (PPARα), exhibiting an EC50 value of 120 nM in a transactivation assay.4 Systemic administration of OEA suppresses food intake and reduces weight gain in rats (10 mg/kg intraperitoneally) and PPARα wild-type mice, but not in PPARα knockout mice.3,4 These data indicate that OEA regulates food intake by a PPARα-mediated mechanism.
1.di Tomaso, E., Beltramo, M., and Piomelli, D.Brain cannabinoids in chocolateNature382677-678(1996)
2.Epps, D.E., Palmer, J.W., Schmid, H.H.O., et al.Inhibition of permeability-dependent Ca2+ release from mitochondria by N-acelethanolamines, a class of lipids synthesized in ischemic heart tissueThe Journal of Biological Chemisty2571383-1392(1982)
3.de Fonseca, F.R., Navarro, M., GÓmez, R., et al.An anorexic lipid mediator regulated by feedingNature414209-212(2001)
4.Fu, J., Gaetani, S., Oveisi, F., et al.Oleylethanolamide regulates feeding and body weight through activation of the nuclear receptor PPAR-αNature42590-93(2003)
