Neferine (NEF) is a natural bisbenzylisoquinoline alkaloid separated from the traditional Chinese medicine Nelumbinis plumula. Neferine has a variety of pharmacological properties[1].
In vitro, Neferine (1, 3 and 10μM; 20min) inhibited the expression of cytokines and chemokines in TNF-α/IFN-γ-stimulated human keratinocyte (HaCaT) cells, and Neferine reduced the phosphorylation of MAPK and the NF-κB signaling pathway[2]. Neferine (12.5 or 25μM; 24h) enhanced the AMPK and ACC phosphorylation in OA-stimulated HepG2 cells [3].
In vivo, Neferine significantly alleviated DNCB-induced skin barrier damage, scratching responses, and epidermal hyperplasia in mice. And Neferine significantly decreased transepidermal water loss (TEWL), erythema, blood flow, and ear thickness and increased surface skin hydration[2]. Neferine (5mg/kg/day or 10mg/kg/day; 4 weeks; i.p.) treatment mitigated hepatic lipid deposition, inflammatory cell infiltration as well as hepatic fibrosis[3]. Neferine (20mg/kg; 30 days; i.p.) inhibits tumorigenesis and induces ferroptosis in nude mice[4].
References:
[1] Marthandam Asokan S, Mariappan R, Muthusamy S, Velmurugan BK. Pharmacological benefits of neferine - A comprehensive review. Life Sci. 2018 Apr 15;199:60-70.
[2] Yang CC, Hung YL, Ko WC, Tsai YJ, Chang JF, Liang CW, Chang DC, Hung CF. Effect of Neferine on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice. Int J Mol Sci. 2021 Jul 30;22(15):8237.
[3] Wang MY, Zhang SS, An MF, Xia YF, Fan MS, Sun ZR, Zhang LJ, Zhao YL, Sheng J, Wang XJ. Neferine ameliorates nonalcoholic steatohepatitis through regulating AMPK pathway. Phytomedicine. 2023 Jun;114:154798.
[4] Li S, Zhang Y, Zhang J, Yu B, Wang W, Jia B, Chang J, Liu J. Neferine Exerts Ferroptosis-Inducing Effect and Antitumor Effect on Thyroid Cancer through Nrf2/HO-1/NQO1 Inhibition. J Oncol. 2022 Jun 27;2022:7933775.
Neferine (NEF)是一种从传统中药莲子心分离出来的天然双苄基异喹啉生物碱。Neferine具有多种药理特性[1]。
在体外，Neferine (1, 3和10μM; 20min)抑制了TNF-α/IFN-γ刺激的人角质形成细胞（HaCaT）中细胞因子和趋化因子的表达，并降低了丝裂原活化蛋白激酶(MAPK)的磷酸化以及核因子κB(NF-κB)信号通路[2]。Neferine (12.5或25μM; 24h)增强了油酸(OA)刺激的HepG2细胞中AMPK和ACC的磷酸化[3]。
在体内，Neferine (3mg/kg和10mg/kg; 15天; 腹腔注射)显著减轻了DNCB诱导的小鼠皮肤屏障损伤、搔抓反应和表皮增生。Neferine显著降低了经表皮失水（TEWL）、红斑、血流和耳厚度，并增加了皮肤表面的水合度[2]。Neferine (5mg/kg/天或10mg/kg/天; 4周; 腹腔注射)治疗减轻了肝脏脂肪沉积、炎性细胞浸润以及肝纤维化[3]。Neferine (20mg/kg; 30天; 腹腔注射)在裸鼠中抑制肿瘤形成并诱导铁死亡[4]。