NADH is the reduced form of nicotinamide adenine dinucleotide (NAD) that can donate electrons as part of a reducing reaction. In that process, NADH becomes oxidized to produce NAD+ . A variety of enzymes use NADH plus H+ to reduce substrates, generating NAD+ as well as the reduced product.1,2,3 For example, NADH:ubiquinone oxidoreductase accepts two electrons from NADH and passes them to ubiquinone (coenzyme Q) as part of the mitochondrial electron transport chain.3
1.Kim, M.S., and Kim, Y.J.Enzymatic properties of the membrane-bound NADH oxidase system in the aerobic respiratory chain of Bacillus cereusJournal of Biochemistry and Molecular Biology37(6)753-756(2004) 2.Godber, B.L.J., Doel, J.J., Sapkota, G.P., et al.Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductaseThe Journal of Biological Chemisty275(11)7757-7763(2000) 3.Fato, R., Bergamini, C., Bortolus, M., et al.Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen speciesBiochim.Biophys.Acta.1787(5)384-392(2009)