ChX710是一种小分子化合物，能够启动I型干扰素对胞质DNA的反应。
Cas No.:2438721-44-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
ChX710 is a small-molecule compound that primes the type I interferon response to cytosolic DNA. ChX710 induces the interferon-stimulated response element (ISRE) promoter, specific interferon-stimulated genes (ISGs), and phosphorylation of interferon regulatory factor (IRF)3, effectively priming the cellular response to DNA transfection via the STING pathway[1].
In vitro, ChX710 (6–50μM) was applied to cell lines such as HEK-293 for 8–48 hours. ChX710 specifically activates the ISRE promoter and induces phosphorylation of IRF3. ChX710 effectively enhances the cellular type I interferon response to cytosolic (transfected) DNA, leading to increased IFN-β secretion. At higher concentrations (25–50µM), ChX710 exhibits significant cytotoxicity, reducing cellular ATP levels and viability[1].
References:
[1] Khiar S, Lucas-Hourani M, Nisole S, et al. Identification of a small molecule that primes the type I interferon response to cytosolic DNA. Sci Rep. 2017 May 31;7(1):2561.
ChX710是一种小分子化合物，能够启动I型干扰素对胞质DNA的反应。ChX710可诱导ISRE启动子序列、特异性细胞干扰素刺激基因（ISGs）和干扰素调节因子（IRF）3的磷酸化，通过STING途径有效启动细胞对DNA转染的反应[1]。
在体外，ChX710（6–50μM）处理HEK-293等细胞系8-48小时。ChX710特异性激活ISRE启动子，可诱导IRF3的磷酸化。ChX710可有效增强细胞对胞质（转染）DNA的I型干扰素反应，导致IFN-β分泌。ChX710在高浓度（25-50µM）下表现出显著的细胞毒性，降低细胞ATP水平和活力[1]。
| Cell experiment [1]: | |
Cell lines | HEK-293T (human embryonic kidney epithelial cells), A549 (human lung epithelial adenocarcinoma cells), MRC5 (human lung fibroblasts), Vero (African green monkey kidney epithelial cells), and primary human peripheral blood mononuclear cells (PBMCs) |
Preparation Method | Reporter cell lines (e.g., STING-37, a HEK-293 cell line stably expressing luciferase under the control of ISRE) or other cell types were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin at 37°C and 5% CO₂. Cells were treated with ChX710 (6-50µM) or related compounds for specified durations. For synergy experiments, cells were co-treated with ChX710 and transfected with plasmid DNA or stimulated with 2',3'-cGAMP using JetPrime transfection reagent. |
Reaction Conditions | 6-50µM; 8-48 hours. |
Applications | ChX710 specifically activates the Interferon-Stimulated Response Element (ISRE) promoter. This activation is dependent on the adaptor protein MAVS and the transcription factor IRF1, but independent of STAT1/STAT2. ChX710 induces phosphorylation of IRF3 but is insufficient to trigger type I interferon (IFN-α/β) secretion on its own. ChX710 induces a modest, cell-type-specific profile of Interferon-Stimulated Genes (ISGs). Crucially, ChX710 potently primes and synergistically enhances the cellular type I interferon response to cytosolic (transfected) DNA, leading to a strong, STING-dependent increase in IFN-β secretion and ISG expression. ChX710 exhibits significant cytotoxicity at higher concentrations, reducing cellular ATP levels and viability. |
References: | |
| Cas No. | 2438721-44-7 | SDF | |
| Canonical SMILES | O=C(NC(C)CN(C)C)C1=C(N=C(C2=CC=NC=C2)N3)C3=CC=C1 | ||
| 分子式 | C18H21N5O | 分子量 | 323.39 |
| 溶解度 | DMSO : 20 mg/mL (61.84 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0922 mL | 15.4612 mL | 30.9224 mL |
| 5 mM | 618.4 μL | 3.0922 mL | 6.1845 mL |
| 10 mM | 309.2 μL | 1.5461 mL | 3.0922 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet