NADA-green is a fluorescent D-amino acid probe suitable for labeling peptidoglycan in living bacteria, with maximum excitation/emission wavelengths of ~450/555nm[1]. NADA-green can be efficiently incorporated into the peptidoglycan layer of different bacteria, strongly leading to peripheral and intermediate labeling of bacterial cell populations without affecting growth rate[2]. NADA-green can be used to detect modified cell wall peptides in E. coli[3]. NADA-green can be used to label living spirochetes[4]. NADA-green can activate the GPCR cannabinoid 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) receptor[5]. NADA probes mainly report L,D-transpeptidase activity, tetrapeptide substrates, or both[6].
References:
[1] Kuru E, Tekkam S, Hall E, et al. Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ[J]. Nature protocols, 2015, 10(1): 33-52.
[2] Arend K I, Schmidt J J, Bentler T, et al. Myxococcus xanthus predation of Gram-positive or Gram-negative bacteria is mediated by different bacteriolytic mechanisms[J]. Applied and Environmental Microbiology, 2021, 87(5): e02382-20.
[3] Kuru E, Hughes H V, Brown P J, et al. In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D‐amino acids[J]. Angewandte Chemie, 2012, 124(50): 12687-12691.
[4] Jutras B L, Scott M, Parry B, et al. Lyme disease and relapsing fever Borrelia elongate through zones of peptidoglycan synthesis that mark division sites of daughter cells[J]. Proceedings of the National Academy of Sciences, 2016, 113(33): 9162-9170.
[5] Fawley J A, Hofmann M E, Andresen M C. Cannabinoid 1 and transient receptor potential vanilloid 1 receptors discretely modulate evoked glutamate separately from spontaneous glutamate transmission[J]. Journal of Neuroscience, 2014, 34(24): 8324-8332.
[6] García-Heredia A, Pohane A A, Melzer E S, et al. Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. eLife, 7[J]. e, 2018, 37243.
NADA-green是一种荧光D-氨基酸探针，适用于标记活细菌中的肽聚糖，最大激发/发射波长~450/555nm[1]。NADA-green能够被有效地掺入到不同细菌的肽聚糖层中，强烈导致细菌细胞群体的外围和中间标记，而不会影响生长速率[2]。NADA-green能够用于检测大肠杆菌中修饰的胞壁肽[3]。NADA-green能够用于标记活螺旋体[4]。NADA-green能够激活 GPCR大麻素1（CB1）和瞬时受体电位香草素1（TRPV1）受体[5]。NADA探针主要报告L,D-转肽酶活性、四肽底物或两者兼有[6]。