N6-methyladenosine (m6A) is a potential agonist of adenosine with an ED50 value of 17250nM[1]. N6-methyladenosine is the methylation occurring at the N6 position of adenine, and is the most common internal modification on eukaryotic mRNA[2]. N6-methyladenosine regulates gene expression, thereby controlling a series of processes such as cell self-renewal, differentiation, invasion and apoptosis[3]. A global decrease in N6-methyladenosine (m6A) results in double-stranded RNA (dsRNA) formation and a profound cell-intrinsic interferon response[4]. The changes in N6-methyladenosine levels are closely related to the clinical and pathological features of gastrointestinal tumors and liver cancer. N6-methyladenosine has become a potential diagnostic and prognostic marker or a promising therapeutic target[5].
References:
[1] Ribeiro J A, Sebastião A M. On the type of receptor involved in the inhibitory action of adenosine at the neuromuscular junction[J]. British journal of pharmacology, 1985, 84(4): 911.
[2] Chen M, Wong C M. The emerging roles of N6-methyladenosine (m6A) deregulation in liver carcinogenesis[J]. Molecular cancer, 2020, 19(1): 44.
[3] He L, Li H, Wu A, et al. Functions of N6-methyladenosine and its role in cancer[J]. Molecular cancer, 2019, 18(1): 176.
[4] Guirguis A A, Ofir-Rosenfeld Y, Knezevic K, et al. Inhibition of METTL3 results in a cell-intrinsic interferon response that enhances antitumor immunity[J]. Cancer Discovery, 2023, 13(10): 2228-2247.
[5] Zhu D H, Su K K, Ou-Yang X X, et al. Mechanisms and clinical landscape of N6-methyladenosine (m6A) RNA modification in gastrointestinal tract cancers[J]. Molecular and Cellular Biochemistry, 2024, 479(7): 1553-1570.
N6-methyladenosine (m6A)是腺苷的潜在激动剂，ED50值为17250nM[1]。作为腺嘌呤N6位点的甲基化修饰，N6-methyladenosine是真核生物mRNA中最常见的内源性化学修饰[2]。N6-methyladenosine通过调控基因表达，参与细胞自我更新、分化、侵袭和凋亡等一系列生物学过程[3]。整体性N6-methyladenosine水平降低会导致双链RNA（dsRNA）形成并引发强烈的细胞内在干扰素反应[4]。N6-methyladenosine水平变化与胃肠道肿瘤和肝癌的临床病理特征密切相关，已成为潜在的诊断预后标志物和治疗靶点[5]。