MOCAc-PLGL(Dpa)AR is a fluorescent substrate specifically designed for the detection of matrix metalloproteinases (MMPs), which is suitable for the activity of MMP-2, MMP-7 and MMP-9[1]. After hydrolysis of MOCAc-PLGL(Dpa)AR, the fluorescence intensity changed at the excitation wavelength of 328nm and the emission wavelength of 393nm[2]. MOCAc-PLGL(Dpa)AR has been widely used as a fluorescent indicator to study the inhibitory effect of alcohols on protease activity of thermophilic bacteria[3].
References:
[1] Muta Y, Yasui N, Matsumiya Y, et al. Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7)[J]. Bioscience, biotechnology, and biochemistry, 2010, 74(12): 2515-2517.
[2] Muta Y, Oneda H, Inouye K. Anomalous pH-dependence of the activity of human matrilysin (matrix metalloproteinase-7) as revealed by nitration and amination of its tyrosine residues[J]. Biochemical Journal, 2005, 386(2): 263-270.
[3] Muta Y, Inouye K. Inhibitory effects of alcohols on thermolysin activity as examined using a fluorescent substrate[J]. The Journal of Biochemistry, 2002, 132(6): 945-951.
MOCAc-PLGL(Dpa)AR是一种专为检测基质金属蛋白酶设计的荧光底物，适用于MMP-2、MMP-7和MMP-9的活性测定[1]。MOCAc-PLGL(Dpa)AR水解后，在328nm激发波长和393 nm发射波长下可观测到荧光强度变化[2]。MOCAc-PLGL(Dpa)AR已作为荧光指示剂广泛应用于研究醇类对嗜热菌蛋白酶活性的抑制作用[3]。