Methylprednisolone is a corticosteroid that interacts with the glucocorticoid receptor and inhibits cortisol secretion with an IC50 of 1.7ng/ml [1]. Methylprednisolone exerts anti-inflammatory effects by inhibiting NF-kappaB and can be utilized in the treatment of tuberculosis and COVID-19 [2].
Methylprednisolone (0.5µM, 5µM, 30µM, or 50µM) could reduce HOG cell proliferation and promote HOG death in a dose-dependent manner. Methylprednisolone also decreased the expression of MBP, a marker for early mature oligodendrocytes, and inhibited myelin regeneration [3].
Methylprednisolone (25mg/kg) treatment significantly ameliorated l Aβ1-42-induced cognitive deficits in Alzheimer's disease mice as well as reduced microglia hyperactivation. Synaptic function-related genes could be upregulated by Methylprednisolone, while immune response-related genes were suppressed by Methylprednisolone. Methylprednisolone alleviated cognitive dysfunction by improving synaptic function and suppressing immune and inflammatory processes [4].
References:
[1]. Lew KH, Ludwig EA, Milad MA, Donovan K, Middleton E Jr, Ferry JJ, Jusko WJ. Gender-based effects on methylprednisolone pharmacokinetics and pharmacodynamics. Clin Pharmacol Ther. 1993 Oct;54(4):402-14. doi: 10.1038/clpt.1993.167. PMID: 8222483; PMCID: PMC4207261.
[2]. Li A, He Y, Yang C, Lu N, Bao J, Gao S, Hosyanto FF, He X, Fu H, Yan H, Ding N, Xu L. Methylprednisolone Promotes Mycobacterium smegmatis Survival in Macrophages through NF-κB/DUSP1 Pathway. Microorganisms. 2023 Mar 16;11(3):768. doi: 10.3390/microorganisms11030768. PMID: 36985341; PMCID: PMC10058212.
[3]. GÓmez-Pinedo U, MatÍas-Guiu JA, Ojeda-Hernandez D, de la Fuente-Martin S, Kamal OM, Benito-Martin MS, Selma-Calvo B, Montero-Escribano P, MatÍas-Guiu J. In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies. Cells. 2023 May 30;12(11):1515. doi: 10.3390/cells12111515. PMID: 37296635; PMCID: PMC10252523.
[4]. Sun Y, Li J, A N, Li Z, Zhong W, Chen L, Liu S, Zhang B, Zhu Z, Li X. Methylprednisolone alleviates cognitive functions through the regulation of neuroinflammation in Alzheimer's disease. Front Immunol. 2023 May 1;14:1192940. doi: 10.3389/fimmu.2023.1192940. PMID: 37197654; PMCID: PMC10183590.
甲泼尼龙是皮质类固醇,可作用于糖皮质激素受体并抑制皮质醇分泌,其IC50为1.7ng/ml[1]。甲泼尼龙通过抑制NF-kappaB而发挥抗炎作用,并且可以运用于结核病及COVID-19的治疗[2]。
甲泼尼龙(0.5µM、5µM、30µM或50µM)处理人少突胶质细,可以以剂量依赖的方式减少 HOG 细胞的增殖,促进 HOG 的死亡。甲基强的松龙还降低了早期成熟少突胶质细胞标记物 MBP 的表达,抑制了髓鞘再生[3]。
甲泼尼龙(25mg/kg)治疗显著改善l Aβ1-42诱导的阿尔茨海默病小鼠的认知障碍,以及减少小胶质细胞的过度激活。突触功能相关基因可以被Methylprednisolone上调,而免疫反应相关基因则被Methylprednisolone所抑制。Methylprednisolone通过改善突触功能和抑制免疫和炎症过程来缓解认知功能障碍[4]。