| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 1mg | ¥1,071.00 | 现货 | 1 | |
| 5mg | ¥2,186.00 | 现货 | 1 |
MCLA is a Cypridina luciferin analog that is used to detect superoxide generated by activated leukocytes and macrophages.1 It emits chemiluminescence near 654 nm by reaction with superoxide or singlet oxygen.1
1.Takahashi, A., Nakano, M., Mashiko, S., et al.The first observation of O2- generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndromeFEBS Lett.261(2)369-372(1990)
Kinase experiment: | The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1]. |
References: [1]. Kimura H, et al. Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase inhuman erythrocytes. FEBS Lett. 1988 Nov 7;239(2):347-50. |
