Lucifer Yellow CH is a water-soluble, high-intensity fluorescent dye with maximum excitation/emission at 430nm/540nm, respectively. Lucifer Yellow CH contains a carbodihydrazide (CH) group that can covalently bind to surrounding biomolecules during aldehyde fixation. Lucifer Yellow CH is impermeable to cell membranes at physiological pH and can be loaded into living cells by microinjection, pinocytosis, scraping, or ATP-induced permeabilization. Lucifer Yellow CH can be used as a biological tracer to label neural cells, monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells [1-5].
References:
[1] Stewart WW. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 1978 Jul;14(3):741-59.
[2] Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92.
[3] Takeuchi K, et al. Effect of superoxide derived from lucifer yellow CH on voltage-gated currents of mouse taste budcells. Chem Senses. 2008 Jun;33(5):425-32.
[4] Sun Y, Leong N T, Jiang T, et al. Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo[J]. Elife, 2017, 6: e29140.
[5] Zhang Y, Hou M C, Li J J, et al. Cardiac β‐adrenergic receptor activation mediates distinct and cell type‐dependent changes in the expression and distribution of connexin 43[J]. Journal of Cellular and Molecular Medicine, 2020, 24(15): 8505-8517.
Lucifer Yellow CH是一种水溶性的高强度荧光染料，最大激发/发射分别为430nm/540nm。Lucifer Yellow CH包含一个碳二酰肼（CH）基团，能够在醛固定过程中与周围的生物分子共价结合。Lucifer Yellow CH在生理pH值下不能渗透细胞膜，可通过显微注射、胞饮作用、刮涂或ATP诱导的渗透作用加载到活细胞中。Lucifer Yellow CH可用作生物示踪剂，标记神经细胞，监测神经元分支、再生、间隙连接检测和表征以及细胞的选择性消融[1-5]。