GlpBio

BCECF (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)

目录号: GC30270纯度: >90.00%同义词: 2,7-双(2-羧乙基)-5(6)-羧基荧光素,2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
A fluorescent pH probe
BCECF (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein)
Cas No.: 85138-49-4
规格价格库存数量操作
1mg¥990.00现货
1
5mg¥2,925.00现货
1
10mg¥4,320.00现货
1
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产品描述 Description

BCECF is a fluorescent probe commonly used to measure pH.1 It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm, when excited at 490 nm versus the emission intensity when excited at 440 nm.1 BCECF is membrane impermeable, whereas the acetoxymethyl ester, BCEBF-AM is membrane permeant.

1.O'Connor, N., and Silver, R.B.Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cellsMethods Cell Biol.81415-433(2007)

实验参考方法 Experimental Reference Method

Kinase experiment:

The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1].

References:

[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45.

产品文档 Product Documents

Purity:>90.00%

化学性质Chemical Properties

CAS 号
85138-49-4
同义词
2,7-双(2-羧乙基)-5(6)-羧基荧光素,2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
SMILES
O=C(O)CCC1=CC(C23OC(C4=C2C=CC(C(O)=O)=C4)=O)=C(OC5=C3C=C(CCC(O)=O)C(O)=C5)C=C1O.O=C(O)CCC6=CC(C78OC(C9=C7C=C(C(O)=O)C=C9)=O)=C(OC%10=C8C=C(CCC(O)=O)C(O)=C%10)C=C6O
分子式
C27H20O11
分子量
520.44 g/mol
溶解性
DMSO : 62.5 mg/mL (120.09 mM);Water : < 0.1 mg/mL (insoluble)
保存条件
Store at -20°C, protect from light
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol