GlpBio

Leptomycin B

目录号: GC10954纯度: >99.50%同义词: 来普霉素B; CI 940; LMB
Leptomycin B (LMB)是染色体区域维持1 (CRM1)的抑制剂,CRM1是核蛋白输出蛋白之一。
Leptomycin B
Cas No.: 87081-35-4
规格价格库存数量操作
5ug (in 20ul Ethanol)¥434.00现货
1
10ug (in 40ul Ethanol)¥742.00现货
1
25ug (in 100ul Ethanol)¥1,484.00现货
1
1mg¥1,596.00现货
1
5mg¥6,230.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

产品描述 Description

Leptomycin B (LMB) is an inhibitor of chromosome region maintenance 1 (CRM1) that is one of the nuclear protein exporters[1]. The IC50 value of Leptomycin B is 0.1-10 nM. Leptomycin B has a potent anti-tumor, anti-inflammatory and neuroprotective effects[2-3].

10-100 nM Leptomycin B(0-100 nM; 48 h) significantly reduced the viability of HGC-27 cells[4]. Leptomycin B (0.5nM; 48 h) displayed a synergistic effect on gefitinib-induced cytotoxicity in A549 cells[5].

Leptomycin B(30 mg/ml(100µl); i.c.v; 7days) prevents status epilepticus (SE) -induced vasogenic edema formation accompanied by preservation of blood-brain barrier (BBB) integrity[6]. Leptomycin B(10 µg/mL (20 µM), 1 µg/mL (2 µM), 100 ng/mL (200 nM), or 10 ng/ mL (20 nM)) can effectively inhibit the inflammatory response and the expression of matrix metalloproteinase (MMP)-9 after UV-B irradiation in mice skin[7].

References:
[1]. Scaffidi P, Misteli T, et,al. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature. 2002 Jul 11;418(6894):191-5. doi: 10.1038/nature00858. Erratum in: Nature. 2010 Sep 30;467(7315):622. PMID: 12110890.
[2]. Lu C, Shao C, et,al. Chemotherapeutic sensitization of leptomycin B resistant lung cancer cells by pretreatment with doxorubicin. PLoS One. 2012;7(3):e32895. doi: 10.1371/journal.pone.0032895. Epub 2012 Mar 7. PMID: 22412944; PMCID: PMC3296751.
[3]. Loewe R, Holnthoner W, et,al. Dimethylfumarate inhibits TNF-induced nuclear entry of NF-kappa B/p65 in human endothelial cells. J Immunol. 2002 May 1;168(9):4781-7. doi: 10.4049/jimmunol.168.9.4781. PMID: 11971029.
[4]. Zhu H, Yang Y, et,al. Leptomycin B inhibits the proliferation, migration, and invasion of cultured gastric carcinoma cells. Biosci Biotechnol Biochem. 2020 Feb;84(2):290-296. doi: 10.1080/09168451.2019.1673148. Epub 2019 Oct 16. PMID: 31619134.
[5]. Liu Z, Gao W. Leptomycin B reduces primary and acquired resistance of gefitinib in lung cancer cells. Toxicol Appl Pharmacol. 2017 Nov 15;335:16-27. doi: 10.1016/j.taap.2017.09.017. Epub 2017 Sep 21. PMID: 28942004; PMCID: PMC5643250.
[6]. Kim DS, Min SJ, et,al. Leptomycin B ameliorates vasogenic edema formation induced by status epilepticus via inhibiting p38 MAPK/VEGF pathway. Brain Res. 2016 Nov 15;1651:27-35. doi: 10.1016/j.brainres.2016.09.023. Epub 2016 Sep 19. PMID: 27659963.
[7]. Kobayashi T, Shinkai H. Leptomycin B reduces matrix metalloproteinase-9 expression and suppresses cutaneous inflammation. J Invest Dermatol. 2005 Feb;124(2):331-7. doi: 10.1111/j.0022-202X.2004.23595.x. PMID: 15675951.

Leptomycin B (LMB)是染色体区域维持1 (CRM1)的抑制剂,CRM1是核蛋白输出蛋白之一[1]。Leptomycin B的IC50值为0.1 ~ 10 nM。Leptomycin B具有有效的抗肿瘤、抗炎和神经保护作用[2-3]。

10 ~ 100 nM Leptomycin B(0-100 nM; 48 h)显著降低HGC-27细胞活力[4]。Leptomycin B (0.5nM; 48 h)对吉非替尼Gefitinib诱导的A549细胞毒性具有协同作用[5]。

Leptomycin B(30 mg/ml(100µl); i.c.v; 7days)可以防止癫痫持续状态(SE)诱导的血管源性水肿形成,同时保持血脑屏障(BBB)的完整性[6]。Leptomycin B(10 µg/mL (20 µM), 1 µg/mL (2 µM), 100 ng/mL (200 nM), or 10 ng/ mL (20 nM))能有效抑制UV-B照射后小鼠皮肤的炎症反应和基质金属蛋白酶(MMP)-9的表达[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

HGC-27 cells

Preparation Method

Cells were incubated with 0, 1, 10, or 100 nM of leptomycin B for 48 h.

Reaction Conditions

0-100 nM; 48 h

Applications

10-100 nM Leptomycin B significantly reduced the viability of HGC-27 cells.
Animal experiment [2]:

Animal models

Male Sprague-Dawley (SD) rats (7 weeks old)

Preparation Method

Mice were anesthetized and placed in a stereotaxic frame. A brain infusion kit was implanted into the right lateral ventricle (1 mm posterior; 1.5 mm lateral; 3.5 mm depth to the bregma) and connected to an osmotic pump (1007D) containing: (1) vehicle; (2) LMB (30 mg/ml). The mice were induced to have epileptic reactions after intracerebroventricular drug infusion.

Dosage form

30 mg/ml(100µl); i.c.v; 7days

Applications

Leptomycin B prevents status epilepticus (SE) -induced vasogenic edema formation accompanied by preservation of blood-brain barrier (BBB) integrity.

References:

[1]. Zhu H, Yang Y,et,al. Leptomycin B inhibits the proliferation, migration, and invasion of cultured gastric carcinoma cells. Biosci Biotechnol Biochem. 2020 Feb;84(2):290-296. doi: 10.1080/09168451.2019.1673148. Epub 2019 Oct 16. PMID: 31619134.
[2]. Kim DS, Min SJ, et,al. Leptomycin B ameliorates vasogenic edema formation induced by status epilepticus via inhibiting p38 MAPK/VEGF pathway. Brain Res. 2016 Nov 15;1651:27-35. doi: 10.1016/j.brainres.2016.09.023. Epub 2016 Sep 19. PMID: 27659963.

产品文档 Product Documents

Purity:>99.50%Appearance:A solution in ethanol

化学性质Chemical Properties

CAS 号
87081-35-4
同义词
来普霉素B; CI 940; LMB
化学名
(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-19-((2S,3S)-3-methyl-6-oxo-3,6-dihydro-2H-pyran-2-yl)-8-oxononadeca-2,10,12,16,18-pentaenoic acid
SMILES
O[C@@H]([C@H](C)C([C@@H](/C=C(C)/C=C/C[C@@H](C)/C=C(CC)\C=C\[C@@H]([C@H](C=C1)C)OC1=O)C)=O)[C@@H](C)C/C(C)=C/C(O)=O
分子式
C33H48O6
分子量
540.74 g/mol
溶解性
50mg/mL in Methanol, 50 mg/ml in Ethanol
保存条件
Desiccate at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol