Leptin Human, N82K PEG

目录号: GP20689纯度: >98%
Leptin N82K Human Recombinant, Pegylated

Leptin Human, N82K PEG
规格价格库存数量操作
20μg¥840.00现货
1
100μg¥2,030.00现货
1
1mg¥12,600.00现货
1

文献被引

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    641, 529–536 (2025)
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    618, 1017–1023 (2023)
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    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    388(6745) (2025)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

产品描述 Description

Pegylated Leptin N82K Human Recombinant produced in E.Coli is a single non-glycosilated polypeptide chain containing 146 amino acids, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus acids and having a molecular weight of 35.6kDa. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Pegylated Leptin N82K Human Recombinant was purified by proprietary chromatographic techniques.

产品文档 Product Documents

Product Data

Purity
Greater than 99.0% as determined by:(a)Gel filtration analysis.(b) Analysis by SDS-PAGE.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Shipping Condition
Shipped at Room temp.
Synonyms
OB Protein; Obesity Protein; OBS; Obesity factor.
Solubility
It is recommended to reconstitute the lyophilized Pegylated Leptin N82K in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Pegylated Leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution Pegylated Leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity
Biological Activity is < than 0.1% as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.
Formulation
Lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3 Having 35-40% protein.