LDS 751 is a cell membrane permeable nucleic acid stain used to distinguish intact nucleated cells from anucleated and damaged nucleated cells, and to distinguish different cell types in mixed neutrophils, lymphocytes and monocytes by flow cytometry. The excitation/emission light of LDS 751 is 543/712nm respectively (when bound to DNA). The maximum excitation wavelength of LDS 751 binding to dsDNA is ~543nm, which can be excited by a 488nm argon ion laser. It is particularly suitable for multicolor analysis due to its longer maximum emission wavelength (~712 nm). Binding to dsDNA causes a fluorescence enhancement of ~20 times. Thiazole orange (mainly binds RNA, GC30038) and LDS751 (mainly binds DNA) are used to distinguish red blood cells, platelets, reticulocytes and nucleated cells.
LDS 751是一种细胞膜通透性的核酸染色剂，用于从无核和受损的有核细胞中区分完整的有核细胞，以及在混合的中性粒细胞、淋巴细胞和单核细胞中用流式细胞术区分不同的细胞类型，LDS 751的激发/发射光分别为543/712nm（与DNA结合时）。LDS 751与dsDNA结合的最大激发波长~543nm，能用488nm氩离子激光器来激发，由于具有较长的最大发射波长(~712 nm)而特别适用于多色分析。与dsDNA的结合引起~20倍的荧光增强。噻唑橙(主要结合RNA，GC30038)和LDS751(主要结合DNA)用来区分红细胞、血小板、网织红血球和有核细胞。