L-Sulforaphene is a natural isothiocyanate with anti-inflammatory, anticancer, and antioxidant effects. L-Sulforaphene regulates multiple signaling pathways such as Nrf2 and NF-κB. L-Sulforaphene can be used in research related to various cancers including liver cancer, breast cancer, lung cancer, esophageal cancer, and gastric cancer, as well as cardiovascular diseases[1-4].
In vitro, L-Sulforaphene (5-10μM) was used to treat 3T3-L1 preadipocytes for 2 days during the early stage of adipogenesis. L-Sulforaphene significantly inhibited lipid accumulation and adipocyte differentiation, and reduced the protein and mRNA expression of PPARγ and C/EBPα, while decreasing C/EBPβ protein levels through post-translational degradation[5]. L-Sulforaphene (20–60μM) was used to treat COV362 cells for 24–48 hours. L-Sulforaphene significantly inhibited cell viability and promoted apoptosis, while upregulating the expression of IGF2BP2 and FAS and downregulating the expression of KRT8 and METTL3[6].
In vivo, L-Sulforaphene (10mg/kg; once daily) was subcutaneously injected into PDX SCID mice implanted with esophageal cancer patient tumors for 6 weeks. L-Sulforaphene significantly reduced tumor size[7]. L-Sulforaphene (10mg/kg or 50mg/kg; every other day) was subcutaneously injected into PDX SCID mice for six weeks. L-Sulforaphene significantly reduced tumor volume[8].
References:
[1] Ye Q, Yan T, Shen J, et al. Sulforaphene targets NLRP3 inflammasome to suppress M1 polarization of macrophages and inflammatory response in rheumatoid arthritis. J Biochem Mol Toxicol. 2023 Jul;37(7):e23362.
[2] Zhang G, Jin C, Zhu Y, et al. Sulforaphene inhibits the progression of osteosarcoma via regulating FSTL1/NF-κB pathway. Life Sci. 2020 Dec 15;263:118485.
[3] Liao Y, Yan Q, Cheng T, et al. Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. J Agric Food Chem. 2023 Oct 25;71(42):15538-15552.
[4] Yao H, Du Y, Jiang B, et al. Sulforaphene suppresses RANKL-induced osteoclastogenesis and LPS-induced bone erosion by activating Nrf2 signaling pathway. Free Radic Biol Med. 2023 Oct;207:48-62.
[5] Yang H, Kang MJ, Hur G, et al. Sulforaphene Suppresses Adipocyte Differentiation via Induction of Post-Translational Degradation of CCAAT/Enhancer Binding Protein Beta (C/EBPβ). Nutrients. 2020 Mar 13;12(3):758.
[6] Yu HY, Yang L, Liu YC, et al. Sulforaphene suppressed cell proliferation and promoted apoptosis of COV362 cells in endometrioid ovarian cancer. PeerJ. 2023 Nov 21;11:e16308.
[7] Zhang C, Wu Q, Yao K, et al. Sulforaphene suppresses oesophageal cancer growth through mitogen- and stress-activated kinase 2 in a PDX mouse model. Am J Cancer Res. 2023 Oct 15;13(10):4708-4720.
[8] Zhang C, Zhang J, Wu Q, et al. Sulforaphene induces apoptosis and inhibits the invasion of esophageal cancer cells through MSK2/CREB/Bcl-2 and cadherin pathway in vivo and in vitro. Cancer Cell Int. 2019 Dec 19;19:342.
L-Sulforaphene是一种天然的异硫氰酸酯，具有抗炎、抗癌和抗氧化作用。L-Sulforaphene调控Nrf2、NF-κB等多种信号通路。L-Sulforaphene可用于肝癌、乳腺癌、肺癌、食道癌和前胃癌等多种癌症以及心血管疾病的相关研究[1-4]。
在体外，L-Sulforaphene（5-10μM）在脂肪形成早期阶段处理3T3-L1前脂肪细胞2天。L-Sulforaphene显著抑制脂质积累和脂肪细胞分化，并降低PPARγ和C/EBPα的蛋白和mRNA表达，同时通过翻译后降解降低C/EBPβ蛋白水平[5]。L-Sulforaphene（20–60μM）处理COV362细胞24–48小时。L-Sulforaphene显著抑制细胞活力并促进细胞凋亡，同时上调IGF2BP2和FAS的表达，下调KRT8和METTL3的表达[6]。
在体内，L-Sulforaphene（10mg/kg；每天一次）皮下注射于植入食管癌患者肿瘤的PDX SCID小鼠，持续六周。L-Sulforaphene显著减少了肿瘤大小[7]。L-Sulforaphene（10mg/kg或50mg/kg；每两天一次）皮下注射于PDX SCID小鼠，持续六周。L-Sulforaphene显著减少了肿瘤体积[8]。