L-Leucine-d3 is a deuterated form of L-Leucine that is used for mass spectrometry (MS) quantification of proteins and as a tracer in metabolic studies[1, 2]. L-Leucine is an essential branched-chain amino acid (BCAA) that activates the mammalian target of rapamycin (mTOR) signaling pathway[3]. L-Leucine is an activator of glutamine:6-phosphofructotransferase (GFAT) and can inhibit NO synthesis in endothelial cells[4]. L-Leucine can be used by skeletal muscle to provide energy during exercise[5]. Both L-Leucine and D-Leucine can effectively inhibit epileptic seizures in mice[6].
References:
[1] Pannkuk E L, Laiakis E C, Girgis M, et al. Temporal effects on radiation responses in nonhuman primates: identification of biofluid small molecule signatures by gas chromatography–mass spectrometry metabolomics[J]. Metabolites, 2019, 9(5): 98.
[2] Zhou J, Liang S, Fang L, et al. Quantitative proteomic analysis of HepG2 cells treated with quercetin suggests IQGAP1 involved in quercetin-induced regulation of cell proliferation and migration[J]. OMICS A Journal of Integrative Biology, 2009, 13(2): 93-103.
[3] Duan Y, Li F, Liu H, et al. Nutritional and regulatory roles of leucine in muscle growth and fat reduction[J]. Front Biosci (Landmark Ed), 2015, 20(4): 796-813.
[4] Yang Y, Wu Z, Meininger C J, et al. L-Leucine and NO-mediated cardiovascular function[J]. Amino Acids, 2015, 47: 435-447.
[5] Mero A. Leucine supplementation and intensive training[J]. Sports Medicine, 1999, 27: 347-358.
[6] Hartman A L, Santos P, O'Riordan K J, et al. Potent anti-seizure effects of D-leucine[J]. Neurobiology of Disease, 2015, 82: 46-53.
L-Leucine-d3是L-Leucine的氘代物，用于蛋白质的质谱（MS）定量测定和作为代谢研究中的示踪剂[1, 2]。L-Leucine是一种必需的支链氨基酸（BCAA），可激活哺乳动物雷帕霉素靶蛋白（mTOR）信号通路[3]。L-Leucine是谷氨酰胺：6-磷酸果糖转氨酶（GFAT）的激活剂，能够抑制内皮细胞中NO合成[4]。L-Leucine可以被骨骼肌用来在运动过程中提供能量[5]。L-Leucine和D-Leucine都能够有效抑制小鼠的癫痫发作[6]。