L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) is a vitamin C derivative usually employed as a raw material in cell and tissue therapy[1]. L-Ascorbic Acid 2-phosphate is commonly used as a substrate for alkaline phosphatase assay[2].
In vitro, L-Ascorbic Acid 2-phosphate treatment (1000μM) for 3 weeks significantly stimulated the proliferation of human skin fibroblasts and induced collagen synthesis[3]. L-Ascorbic Acid 2-phosphate treatment (300μM) for 2 weeks significantly stimulated the growth of human corneal endothelial cells, maintained the characteristic polygonal morphology, reduced the generation of 8-hydroxy-2-deoxyguanosine (8-OHdG), and prevented oxidative DNA damage[4]. After 30 days of culture in the presence of L-Ascorbic Acid 2-phosphate (100μM), the growth of rabbit corneal cells was significantly increased, and the cells had multilayer characteristics[5]. L-Ascorbic Acid 2-phosphate (250μM) treatment for 7 days significantly inhibited the growth of adipose-derived stem cells seeded at low density (1,250 cells/cm2), resulting in decreased expression of antioxidant enzymes[6]. L-Ascorbic Acid 2-phosphate treatment (50μM; 24h) significantly and sustainably improved intracellular L-ascorbic acid and enhanced type 1 collagen synthesis and mRNA expression in human gingival fibroblasts, with reduction of intracellular reactive oxygen species (ROS)[7].
References:
[1] Xu X, Woźniczka M, Van Hecke K, et al. Structural study of L-ascorbic acid 2-phosphate magnesium, a raw material in cell and tissue therapy[J]. JBIC Journal of Biological Inorganic Chemistry, 2020, 25(6): 875-885.
[2] Wu Y, Chen W, Wang C, et al. Assays for alkaline phosphatase that use L-ascorbic acid 2-phosphate as a substrate[J]. Coordination Chemistry Reviews, 2023, 495: 215370.
[3] Hata R I, Senoo H. L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts[J]. Journal of cellular physiology, 1989, 138(1): 8-16.
[4] Shima N, Kimoto M, Yamaguchi M, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate[J]. Investigative ophthalmology & visual science, 2011, 52(12): 8711-8717.
[5] Saika S, Kanagawa R, Uenoyama K, et al. L-ascorbic acid 2-phosphate, a phosphate derivative of L-ascorbic acid, enhances the growth of cultured rabbit keratocytes[J]. Graefe's archive for clinical and experimental ophthalmology, 1991, 229(1): 79-83.
[6] Wu Y K, Tu Y K, Yu J, et al. The influence of cell culture density on the cytotoxicity of adipose-derived stem cells induced by L-ascorbic acid-2-phosphate[J]. Scientific Reports, 2020, 10(1): 104.
[7] Tsutsumi K, Fujikawa H, Kajikawa T, et al. Effects of L‐ascorbic acid 2‐phosphate magnesium salt on the properties of human gingival fibroblasts[J]. Journal of periodontal research, 2012, 47(2): 263-271.
L-Ascorbic Acid 2-phosphate (magnesium salt hydrate)是一种常用于细胞和组织疗法原料的维生素C衍生物[1]。L-Ascorbic Acid 2-phosphate常作为碱性磷酸酶检测的底物[2]。
在体外，1000μM浓度的L-Ascorbic Acid 2-phosphate处理3周可显著促进人皮肤成纤维细胞增殖并诱导胶原合成[3]。300μM浓度的L-Ascorbic Acid 2-phosphate处理2周能有效刺激人角膜内皮细胞生长，维持特征性多边形形态，减少8-羟基-2-脱氧鸟苷(8-OHdG)生成，预防氧化DNA损伤[4]。100μM浓度的L-Ascorbic Acid 2-phosphate培养30天使兔角膜细胞生长显著增加，并呈现多层特征[5]。250μM浓度的L-Ascorbic Acid 2-phosphate处理7天会显著抑制低密度接种（1,250细胞/cm2）的脂肪干细胞生长，导致抗氧化酶表达降低[6]。50μM浓度的L-Ascorbic Acid 2-phosphate处理24小时可持续提升人牙龈成纤维细胞内L-抗坏血酸水平，增强I型胶原合成及mRNA表达，同时降低细胞内活性氧(ROS)[7]。