Juvenile Hormone III is an acyclic sesquiterpenoid that regulates diverse processes about adult transition of larvae and oogenesis in adult females. Juvenile Hormone III activates the juvenile hormone receptor with a Kd value of 2.9nM[1]. Juvenile Hormone III can be synthesized from farnesoic acid by two alternative pathways, one through methyl farnesoate and the other through Juvenile Hormone III[2].
Juvenile Hormone III (10μM; 2h) induced TcKr-h1 transcript expression dose-dependently in Tc81 cells; the 50% effective concentration (EC50) were 2.7x10-11M [3]. In Drosophila S2 cells, the kJHRE reporter was activated by Juvenile Hormone III (0.1μM; 24h) when the both TcMet and TcSRC were expressed [3].
Juvenile Hormone III (100, 500ng; topical application) rescues the head eversion defect in tail-ablated (CAX) larvae[4]. Juvenile Hormone III (10μg/larva; topical application) diminished the number of S-phase nuclei in ovaries of late feeding-phase workers, bringing them to queen-like levels and prevented the induction of apoptotic DNA degradation[5].
References:
[1].Charles JP, Iwema T, Epa VC, Takaki K, Rynes J, Jindra M. Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant. Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21128-33.
[2].Defelipe LA, Dolghih E, Roitberg AE, Nouzova M, Mayoral JG, Noriega FG, Turjanski AG. Juvenile hormone synthesis: "esterify then epoxidize" or "epoxidize then esterify" Insights from the structural characterization of juvenile hormone acid methyltransferase. Insect Biochem Mol Biol. 2011 Apr;41(4):228-35.
[3]. Kayukawa T, Tateishi K, Shinoda T. Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum. Sci Rep. 2013;3:1570.
[4]. A role for juvenile hormone in the prepupal development of Drosophila melanogaster. Development. 2010 Apr;137(7):1117-26.
[5]. Capella IC, Hartfelder K. Juvenile hormone effect on DNA synthesis and apoptosis in caste-specific differentiation of the larval honey bee (Apis mellifera L.) ovary. J Insect Physiol. 1998 May;44(5-6):385-391.
Juvenile Hormone III是一种非环状倍半萜类化合物，参与调节幼虫成年转变和成年雌性卵子发生的各种过程。Juvenile Hormone III激活保幼激素受体，Kd值为2.9nM[1]。Juvenile Hormone III可以通过两种替代途径由法尼酸合成，一种是通过法呢酸甲酯，另一种是通过保幼激素酸III[2]。
Juvenile Hormone III（10μM；2h）在Tc81细胞中剂量依赖性地诱导TcKr-h1转录本表达，其EC50为2.7×10−11M[3]。在果蝇S2细胞中，当TcMet和TcSRC均表达时，kJHRE报告基因被Juvenile Hormone III（0.1μM；24h）激活[3]。
Juvenile Hormone III（100，500ng；局部应用）可挽救断尾（CAX）幼虫的头部外翻缺陷[4]。Juvenile Hormone III（10μg/幼虫；局部应用）可减少后期取食期工蜂卵巢中 S 期细胞核的数量，使其降至蜂王水平，并防止诱导细胞凋亡 DNA 降解[5]。