GlpBio

ISX 9

目录号: GC10472纯度: >98.50%同义词: Isoxazole 9
ISX 9 是成体神经干细胞分化的有效诱导剂。
ISX 9
Cas No.: 832115-62-5
规格价格库存数量操作
1mg¥238.00现货
1
5mg¥525.00现货
1
10mg¥770.00现货
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50mg¥1,750.00现货
1
100mg¥3,150.00现货
1
10mM (in 1mL DMSO)¥578.00现货
1
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产品描述 Description

ISX 9 is a potent inducer of adult neural stem cell differentiation[1]. ISX 9 activates Ca2+ influx through both voltage-gated Ca2+ channels and NMDA receptors and increases neuroD expression[2]. ISX 9 is usually used for research related to neurogenesis, neuropsychiatric disorders, and circadian rhythm disorders[3][4].

In vitro, ISX 9 (10μM; 4 days) enhances circadian amplitude in synchronized mouse embryonic fibroblasts[5]. ISX 9 (1.25-5μM; 24-48h) dose-dependently promotes KGF and Ang-1 secretion in mesenchymal stem cells[6].

In vivo, ISX 9 (20mg/kg/day; i.p.; 12 days) reversed chronic intermittent ethanol exposure-induced enhancement of fear memory retrieval and restored structural and functional plasticity of ventral dentate gyrus granule cell neurons in male Wistar rats during protracted abstinence[7].

References:
[1] Schneider JW, Gao Z, Li S, et al. Small-molecule activation of neuronal cell fate. Nat Chem Biol. 2008;4(7):408-410.
[2] Petrik D, Jiang Y, Birnbaum SG, et al. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB J. 2012;26(8):3148-3162.
[3] Bettio LEB, Gil-Mohapel J, Patten AR, et al. Effects of Isx-9 and stress on adult hippocampal neurogenesis: Experimental considerations and future perspectives. Neurogenesis (Austin). 2017;4(1):e1317692.
[4] Koh SH, Liang AC, Takahashi Y, et al. Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells. PLoS One. 2015;10(9):e0138724.
[5] Li H, Ou J, Li Y, et al. ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude. Commun Biol. 2022;5(1):750.
[6] Tian Y, Deng Q, Yang X, et al. ISX-9 Promotes KGF Secretion From MSCs to Alleviate ALI Through NGFR-ERK-TAU-β-Catenin Signaling Axis. Stem Cells Transl Med. 2024;13(3):255-267.
[7] Staples MC, Herman MA, Lockner JW, et al. Isoxazole-9 reduces enhanced fear responses and retrieval in ethanol-dependent male rats. J Neurosci Res. 2021;99(11):3047-3065.

ISX 9 是成体神经干细胞分化的有效诱导剂[1]。ISX 9 通过电压门控钙通道和NMDAR激活钙离子内流，并增加NeuroD表达[2]。ISX 9通常用于神经发生、神经精神疾病及昼夜节律紊乱相关研究[3][4]。

体外实验中，ISX 9（10μM；4天）可增强同步化小鼠胚胎成纤维细胞的昼夜节律振幅[5]。ISX 9（1.25-5μM；24-48小时）剂量依赖性地促进间充质干细胞分泌KGF和Ang-1[6]。

体内实验中，ISX 9（20mg/kg/天；腹腔注射；12天）可在雄性Wistar大鼠的长期乙醇戒断期间逆转慢性间歇性乙醇暴露诱导的恐惧记忆增强，并恢复腹侧齿状回颗粒细胞神经元的结构和功能可塑性[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

MEF cells

Preparation Method

For the real-time recording of PER2::LUC bioluminescence, 2.5×105 mPer2Luc MEF cells were seeded and then cultured for 48h with DMEM in 3.5cm dishes until confluent. The cells were then synchronized with 200nM dexamethasone for 1h, before the bioluminescence recording in serum-free DMEM medium supplemented with 200μM luciferin together with ISX 9 at 10μM. The cultures were recorded in a light-tight chamber containing photon multiplier tube recording (PMT) detector assemblies for continuous 4 days. Data including amplitude and period results, were processed with the LumiCycle Analysis software. Cell cultures were lysed for immunoblot analysis.

Reaction Conditions

10μM; 4 days

Applications

ISX 9 increased PER2::LUC oscillations and DBP protein levels in synchronized MEF cells.
Animal experiment [2]:

Animal models

male Wistar rats

Preparation Method

Two hundred and thirteen adult male Wistar rats were housed in a temperature-controlled (22°C) vivarium with ad libitum access to food and water and completed the study. Animals were set on a 12-hr light/ 12-hr dark cycle, with the light cycle beginning at 8:00p.m., in groups of two or three animals per cage unit. During chronic intermittent ethanol vapor exposure (CIE), 106 rats were exposed to cycles of ethanol vapor produced via vaporization of 95% ethanol in a heated flask that was immediately conveyed through controlled air flow to rat vapor chambers on a 14-hr on/10-hr off daily schedule for the duration of 7 weeks. The vapor flow rate was calibrated so as to achieve target blood alcohol levels (BALs) within the range of 125-250mg/dl. BALs were measured utilizing an Analox AM1 analyzer. Following 7 weeks of CIE, rats were forced withdrawn from CIE and experienced training of trace fear conditioning (TFC). A subset of these rats were tested for context and fear retrieval at three time points post training-early (24h), mid (10 days), and late abstinence (21 days). The remaining 107 rats served as air controls (Air; no CIE) and were housed in similar cages as the CIE rats without the vapor exposure. ISX 9 was prepared for injections with 25% HBC (2-hydrox ypropyl-β-cyclodextrin). Following 5 weeks of CIE, a subset of CIE (n=39) and Air (n=40) rats were injected with ISX 9 (i.p.; 20mg/kg body weight) once per day for 12 days. Subsequently, a subset of animals (21-day time point; n=20 Air, n=19 CIE) received one 5′-bromo-2-deoxyuridine (BrdU) injection (i.p.; 150mg/kg). Forty-eight hours after the last ISX 9 injection, or 72 hr after the last vapor session, CIE and Air rats were trained for Trace fear conditioning.

Dosage form

20mg/kg/day; i.p.; 12 days

Applications

ISX 9 reversed chronic intermittent ethanol exposure-induced enhancement of fear memory retrieval and restored structural and functional plasticity of ventral dentate gyrus granule cell neurons in male Wistar rats during protracted abstinence.

References:
[1] Li H, Ou J, Li Y, et al. ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude. Commun Biol. 2022;5(1):750.
[2] Staples MC, Herman MA, Lockner JW, et al. Isoxazole-9 reduces enhanced fear responses and retrieval in ethanol-dependent male rats. J Neurosci Res. 2021;99(11):3047-3065.

产品文档 Product Documents

Purity:>98.50%

化学性质Chemical Properties

CAS 号
832115-62-5
同义词
Isoxazole 9
化学名
N-cyclopropyl-5-(thiophen-2-yl)isoxazole-3-carboxamide
SMILES
O=C(C1=NOC(C2=CC=CS2)=C1)NC3CC3
分子式
C11H10N2O2S
分子量
234.27 g/mol
溶解性
≥ 23.4mg/mL in DMSO
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol