ISX 9 是成体神经干细胞分化的有效诱导剂。
Cas No.:832115-62-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
ISX 9 is a potent inducer of adult neural stem cell differentiation[1]. ISX 9 activates Ca2+ influx through both voltage-gated Ca2+ channels and NMDA receptors and increases neuroD expression[2]. ISX 9 is usually used for research related to neurogenesis, neuropsychiatric disorders, and circadian rhythm disorders[3][4].
In vitro, ISX 9 (10μM; 4 days) enhances circadian amplitude in synchronized mouse embryonic fibroblasts[5]. ISX 9 (1.25-5μM; 24-48h) dose-dependently promotes KGF and Ang-1 secretion in mesenchymal stem cells[6].
In vivo, ISX 9 (20mg/kg/day; i.p.; 12 days) reversed chronic intermittent ethanol exposure-induced enhancement of fear memory retrieval and restored structural and functional plasticity of ventral dentate gyrus granule cell neurons in male Wistar rats during protracted abstinence[7].
References:
[1] Schneider JW, Gao Z, Li S, et al. Small-molecule activation of neuronal cell fate. Nat Chem Biol. 2008;4(7):408-410.
[2] Petrik D, Jiang Y, Birnbaum SG, et al. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB J. 2012;26(8):3148-3162.
[3] Bettio LEB, Gil-Mohapel J, Patten AR, et al. Effects of Isx-9 and stress on adult hippocampal neurogenesis: Experimental considerations and future perspectives. Neurogenesis (Austin). 2017;4(1):e1317692.
[4] Koh SH, Liang AC, Takahashi Y, et al. Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells. PLoS One. 2015;10(9):e0138724.
[5] Li H, Ou J, Li Y, et al. ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude. Commun Biol. 2022;5(1):750.
[6] Tian Y, Deng Q, Yang X, et al. ISX-9 Promotes KGF Secretion From MSCs to Alleviate ALI Through NGFR-ERK-TAU-β-Catenin Signaling Axis. Stem Cells Transl Med. 2024;13(3):255-267.
[7] Staples MC, Herman MA, Lockner JW, et al. Isoxazole-9 reduces enhanced fear responses and retrieval in ethanol-dependent male rats. J Neurosci Res. 2021;99(11):3047-3065.
ISX 9 是成体神经干细胞分化的有效诱导剂[1]。ISX 9 通过电压门控钙通道和NMDAR激活钙离子内流，并增加NeuroD表达[2]。ISX 9通常用于神经发生、神经精神疾病及昼夜节律紊乱相关研究[3][4]。
体外实验中，ISX 9（10μM；4天）可增强同步化小鼠胚胎成纤维细胞的昼夜节律振幅[5]。ISX 9（1.25-5μM；24-48小时）剂量依赖性地促进间充质干细胞分泌KGF和Ang-1[6]。
体内实验中，ISX 9（20mg/kg/天；腹腔注射；12天）可在雄性Wistar大鼠的长期乙醇戒断期间逆转慢性间歇性乙醇暴露诱导的恐惧记忆增强，并恢复腹侧齿状回颗粒细胞神经元的结构和功能可塑性[7]。
| Cell experiment [1]: | |
Cell lines | MEF cells |
Preparation Method | For the real-time recording of PER2::LUC bioluminescence, 2.5×105 mPer2Luc MEF cells were seeded and then cultured for 48h with DMEM in 3.5cm dishes until confluent. The cells were then synchronized with 200nM dexamethasone for 1h, before the bioluminescence recording in serum-free DMEM medium supplemented with 200μM luciferin together with ISX 9 at 10μM. The cultures were recorded in a light-tight chamber containing photon multiplier tube recording (PMT) detector assemblies for continuous 4 days. Data including amplitude and period results, were processed with the LumiCycle Analysis software. Cell cultures were lysed for immunoblot analysis. |
Reaction Conditions | 10μM; 4 days |
Applications | ISX 9 increased PER2::LUC oscillations and DBP protein levels in synchronized MEF cells. |
| Animal experiment [2]: | |
Animal models | male Wistar rats |
Preparation Method | Two hundred and thirteen adult male Wistar rats were housed in a temperature-controlled (22°C) vivarium with ad libitum access to food and water and completed the study. Animals were set on a 12-hr light/ 12-hr dark cycle, with the light cycle beginning at 8:00p.m., in groups of two or three animals per cage unit. During chronic intermittent ethanol vapor exposure (CIE), 106 rats were exposed to cycles of ethanol vapor produced via vaporization of 95% ethanol in a heated flask that was immediately conveyed through controlled air flow to rat vapor chambers on a 14-hr on/10-hr off daily schedule for the duration of 7 weeks. The vapor flow rate was calibrated so as to achieve target blood alcohol levels (BALs) within the range of 125-250mg/dl. BALs were measured utilizing an Analox AM1 analyzer. Following 7 weeks of CIE, rats were forced withdrawn from CIE and experienced training of trace fear conditioning (TFC). A subset of these rats were tested for context and fear retrieval at three time points post training-early (24h), mid (10 days), and late abstinence (21 days). The remaining 107 rats served as air controls (Air; no CIE) and were housed in similar cages as the CIE rats without the vapor exposure. ISX 9 was prepared for injections with 25% HBC (2-hydrox ypropyl-β-cyclodextrin). Following 5 weeks of CIE, a subset of CIE (n=39) and Air (n=40) rats were injected with ISX 9 (i.p.; 20mg/kg body weight) once per day for 12 days. Subsequently, a subset of animals (21-day time point; n=20 Air, n=19 CIE) received one 5′-bromo-2-deoxyuridine (BrdU) injection (i.p.; 150mg/kg). Forty-eight hours after the last ISX 9 injection, or 72 hr after the last vapor session, CIE and Air rats were trained for Trace fear conditioning. |
Dosage form | 20mg/kg/day; i.p.; 12 days |
Applications | ISX 9 reversed chronic intermittent ethanol exposure-induced enhancement of fear memory retrieval and restored structural and functional plasticity of ventral dentate gyrus granule cell neurons in male Wistar rats during protracted abstinence. |
References: | |
| Cas No. | 832115-62-5 | SDF | |
| 别名 | Isoxazole 9 | ||
| 化学名 | N-cyclopropyl-5-(thiophen-2-yl)isoxazole-3-carboxamide | ||
| Canonical SMILES | O=C(C1=NOC(C2=CC=CS2)=C1)NC3CC3 | ||
| 分子式 | C11H10N2O2S | 分子量 | 234.27 |
| 溶解度 | ≥ 23.4mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.2686 mL | 21.3429 mL | 42.6858 mL |
| 5 mM | 853.7 μL | 4.2686 mL | 8.5372 mL |
| 10 mM | 426.9 μL | 2.1343 mL | 4.2686 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet