Isovitexin 2''-O-arabinoside是来自Avena sativa L.的C-糖基黄酮，具有植物毒性活性。
Cas No.:53382-71-1
Sample solution is provided at 25 µL, 10mM.
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Isovitexin 2''-O-arabinoside is the C-glycosylflavone from Avena sativa L. with Phytotoxic activity [1]. Isovitexin 2''-O-arabinoside is capable of effectively reducing the accumulation of intracellular reactive oxygen species (ROS), activating the PI3K/NQO-1 pathway, thereby enhancing the antioxidant response[2]. Isovitexin 2''-O-arabinoside is widely used as an internal reference to optimize chromatographic purification conditions and nuclear magnetic resonance parameters for the efficient purification and identification of related compounds[3].
In vitro, Isovitexin 2''-O-arabinoside treatment (10μM) for 3 days significantly enhanced the alkaline phosphatase (ALP) activity in BMP-2-stimulated C2C12 cells and promoted cell differentiation[4].
References:
[1] De Bertoldi C, De Leo M, Braca A, et al. Bioassay-guided isolation of allelochemicals from Avena sativa L.: allelopathic potential of flavone C-glycosides[J]. Chemoecology, 2009, 19(3): 169-176.
[2] Huang M, Chen Q, Li S, et al. Germination-driven dynamic changes of phenolic compounds in oats and the underlying mechanisms for antioxidation enhancement[J]. Food Chemistry, 2025: 146344.
[3] Zhou G, Wu H, Wang T, et al. C-glycosylflavone with rotational isomers from Vaccaria hispanica (Miller) Rauschert seeds[J]. Phytochemistry letters, 2017, 19: 241-247.
[4] Woo S Y, Lee K S, Shin H L, et al. Two new secondary metabolites isolated from Avena sativa L.(Oat) seedlings and their effects on osteoblast differentiation[J]. Bioorganic & Medicinal Chemistry Letters, 2020, 30(14): 127250.
Isovitexin 2''-O-arabinoside是来自Avena sativa L.的C-糖基黄酮，具有植物毒性活性[1]。Isovitexin 2''-O-arabinoside能够有效减少细胞内活性氧的积累，激活PI3K/NQO-1通路，从而增强抗氧化反应[2]。Isovitexin 2''-O-arabinoside被广泛用作内参，以优化色谱纯化条件和核磁共振参数，从而高效纯化和鉴定相关化合物[3]。
在体外，10μM的Isovitexin 2''-O-arabinoside处理BMP-2刺激的C2C12细胞3天，显著增强了碱性磷酸酶（ALP）活性并促进了细胞分化[4]。
| Cell experiment [1]: | |
Cell lines | C2C12 cells |
Preparation Method | C2C12 cells were incubated in alpha minimum essential medium (α‐MEM) that contained 10% FBS, 100U/ml penicillin, and 100μg/ml streptomycin in a humidified incubator at 37°C and 5% CO2. For differentiation into osteoblasts, the C2C12 cells were seeded and allowed to attach and grow for one day. After differentiating for three days by BMP-2 (100ng/ml) in the presence of Isovitexin 2''-O-arabinoside (0.3, 1, 3, and 10μM), the cells were washed twice with PBS, fixed with 10% formalin in PBS for 5 min, and rinsed with deionized water. The ALP staining and activity assays were conducted. |
Reaction Conditions | 0.3, 1, 3, and 10μM; 72h |
Applications | Isovitexin 2''-O-arabinoside treatment significantly enhanced ALP activity in C2C12 cells in a concentration-dependent manner. |
| Cas No. | 53382-71-1 | SDF | |
| 别名 | 异牡荆素-2''-O-阿拉伯糖苷 | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(O)=C([C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O[C@H]5[C@@H]([C@H]([C@H](CO5)O)O)O)C(O)=C13 | ||
| 分子式 | C26H28O14 | 分子量 | 564.49 |
| 溶解度 | DMSO : 100 mg/mL (177.15 mM; Need ultrasonic) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7715 mL | 8.8576 mL | 17.7151 mL |
| 5 mM | 354.3 μL | 1.7715 mL | 3.543 mL |
| 10 mM | 177.2 μL | 885.8 μL | 1.7715 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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