Isovitexin 2''-O-arabinoside is the C-glycosylflavone from Avena sativa L. with Phytotoxic activity [1]. Isovitexin 2''-O-arabinoside is capable of effectively reducing the accumulation of intracellular reactive oxygen species (ROS), activating the PI3K/NQO-1 pathway, thereby enhancing the antioxidant response[2]. Isovitexin 2''-O-arabinoside is widely used as an internal reference to optimize chromatographic purification conditions and nuclear magnetic resonance parameters for the efficient purification and identification of related compounds[3].
In vitro, Isovitexin 2''-O-arabinoside treatment (10μM) for 3 days significantly enhanced the alkaline phosphatase (ALP) activity in BMP-2-stimulated C2C12 cells and promoted cell differentiation[4].
References:
[1] De Bertoldi C, De Leo M, Braca A, et al. Bioassay-guided isolation of allelochemicals from Avena sativa L.: allelopathic potential of flavone C-glycosides[J]. Chemoecology, 2009, 19(3): 169-176.
[2] Huang M, Chen Q, Li S, et al. Germination-driven dynamic changes of phenolic compounds in oats and the underlying mechanisms for antioxidation enhancement[J]. Food Chemistry, 2025: 146344.
[3] Zhou G, Wu H, Wang T, et al. C-glycosylflavone with rotational isomers from Vaccaria hispanica (Miller) Rauschert seeds[J]. Phytochemistry letters, 2017, 19: 241-247.
[4] Woo S Y, Lee K S, Shin H L, et al. Two new secondary metabolites isolated from Avena sativa L.(Oat) seedlings and their effects on osteoblast differentiation[J]. Bioorganic & Medicinal Chemistry Letters, 2020, 30(14): 127250.
Isovitexin 2''-O-arabinoside是来自Avena sativa L.的C-糖基黄酮，具有植物毒性活性[1]。Isovitexin 2''-O-arabinoside能够有效减少细胞内活性氧的积累，激活PI3K/NQO-1通路，从而增强抗氧化反应[2]。Isovitexin 2''-O-arabinoside被广泛用作内参，以优化色谱纯化条件和核磁共振参数，从而高效纯化和鉴定相关化合物[3]。
在体外，10μM的Isovitexin 2''-O-arabinoside处理BMP-2刺激的C2C12细胞3天，显著增强了碱性磷酸酶（ALP）活性并促进了细胞分化[4]。