Isoshaftoside is a C-glycosylflavonoid and can be isolated from plants like Passiflora incarnata L., Viola yedoensis Makino, and Abrus cantoniensis, and it can inhibit the growth of germinating S. hermonthica radicles[1][2][3]. Isoshaftoside exert several pharmacological effects, such as anti inflammatory[4] and antioxidant effects[5].
Isoshaftoside (1µM; 12d) treatment can restore mitochondrial function and reduce glycolysis dependence in human dermal fibroblasts[6]. In HepG2 cells, treatment with Isoshaftoside at 300µM for 24 hours reduced the expression levels of LC3-II and p62[7].
Isoshaftoside (20mg/kg/day; 4 weeks; ip) treatment can reduce the body weight of high-fat diet mice and alleviate hepatic steatosis in nonalcoholic fatty liver disease(NAFLD)[7]. Isoshaftoside (50µM; 12 weeks; oral) treatment significantly decreased lobular inflammation fibrosis induced by a high-fat diet[8].
References:
[1]. Toda K, Hitoe S, Takeda S, et al. Passionflower Extract Induces High-amplitude Rhythms without Phase Shifts in the Expression of Several Circadian Clock Genes in Vitro and in Vivo. Int J Biomed Sci. 2017 Jun;13(2):84-92. PMID: 28824345; PMCID: PMC5542920.
[2]. Hu XL, Niu YJ, Chen M, et al. Preventive Effects of Total Flavonoid C-Glycosides from Abrus mollis on Nonalcoholic Fatty Liver Disease through Activating the PPARα Signaling Pathway. Planta Med. 2019 May;85(8):678-688. doi: 10.1055/a-0895-5838. Epub 2019 Apr 26. PMID: 31026873.
[3]. Hooper AM, Tsanuo MK, Chamberlain K, et al. Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, is an allelochemical against the development of Striga. Phytochemistry. 2010 Jun;71(8-9):904-8. doi: 10.1016/j.phytochem.2010.02.015. Epub 2010 Mar 6. PMID: 20211477.
[4]. Guan S, Sun L, Wang X, et al. Isoschaftoside Inhibits Lipopolysaccharide-Induced Inflammation in Microglia through Regulation of HIF-1α-Mediated Metabolic Reprogramming. Evid Based Complement Alternat Med. 2022 Nov 23;2022:5227335. doi: 10.1155/2022/5227335. PMID: 36467557; PMCID: PMC9711954.
[5]. Congyou DENG, Xinhe LEI, Minyou HE, et al. Identifying the active antioxidant ingredients in Arisaema Cum Bile aqueous extract on the basis of spectrum-effect relationships. Chinese Journal of Analytical Chemistry,Volume 52, Issue 12,2024,100453,ISSN 1872-2040.https://doi.org/10.1016/j.cjac.2024.100453.
[6]. Lee YH, So BH, Lee KS, et al. Identification of Cellular Isoschaftoside-Mediated Anti-Senescence Mechanism in RAC2 and LINC00294. Molecules. 2024 Sep 4;29(17):4182. doi: 10.3390/molecules29174182. PMID: 39275030; PMCID: PMC11397025.
[7]. Su Y, Kang Y, Yi J, et al. Isoschaftoside Reverses Nonalcoholic Fatty Liver Disease via Activating Autophagy In Vivo and In Vitro. Evid Based Complement Alternat Med. 2022 Jun 27;2022:2122563. doi: 10.1155/2022/2122563. PMID: 35795282; PMCID: PMC9252632.
[8]. Abe T. Isoschaftoside in Fig Leaf Tea Alleviates Nonalcoholic Fatty Liver Disease in Mice via the Regulation of Macrophage Polarity. Nutrients. 2025 Feb 21;17(5):757. doi: 10.3390/nu17050757. PMID: 40077628; PMCID: PMC11902273.
Isoshaftoside是一种C-糖苷黄酮类化合物，可从西番莲、紫花地丁和广东相思子等植物中分离出来，能够抑制发芽的独脚金根尖的生长[1][2][3]。Isoshaftoside具有多种药理作用，如抗炎[4]和抗氧化作用[5]。
Isoshaftoside（1μM；12天）处理可以恢复人皮肤成纤维细胞的线粒体功能并减少其对糖酵解的依赖[6]。在HepG2细胞中，用300μM Isoshaftoside处理24小时可降低LC3-II和p62的表达水平[7]。
Isoshaftoside（20mg/kg/天；4周；腹腔注射）处理可以减少高脂饮食小鼠的体重并减轻非酒精性脂肪性肝病（NAFLD）中的肝脂肪变性[7]。Isoshaftoside（50μM；12周；口服）处理显著减少高脂饮食引起的肝小叶炎症和纤维化[8]。