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Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. The IL-4 is purified by proprietary chromatographic techniques.
| Purity | > 95%, determined by SDS-PAGE. | Source | CHO |
| Phycical Appearance | Solution Protein. | Shipping Condition | Shipped at Room temp. |
| Synonyms | BCGF; BCDF; B cell stimulating factor; BSF-1; Lymphocyte stimulatory factor 1; IL-4; MGC79402; Binetrakin; Pitrakinra. | ||
| Amino Acid Sequence | MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVCRASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNESKSTSLKDFLE SLKSIMQMDY S. | ||
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg. | ||
| Formulation | PBS solution with protein concentration of 0.2 mg/mL.This solution can be diluted into other aqueous buffers. | ||
Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.
The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg.
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.