Hoechst 34580是一种DNA染料，最大激发波长为368nm，最大发射波长为437nm[1]。Hoechst 34580结合于DNA小沟的A–T富集区，可对活细胞和固定细胞的DNA进行染色，并兼容免疫组织化学应用[2]。Hoechst 34580能够穿过血脑屏障，并以0.86µM的IC50值抑制Aβ42的聚集[3]。Hoechst 34580可用于开发一种检测恶性疟原虫感染红细胞的荧光蓝光光学系统[4]。
Fig. Fluorescence excitation and emission spectra of Hoechst 34580 bound to DNA
References:
[1] Selph K E. Enumeration of marine microbial organisms by flow cytometry using near‐UV excitation of Hoechst 34580‐stained DNA[J]. Limnology and Oceanography: Methods, 2021, 19(10): 692-701.
[2] Bucevičius J, Lukinavičius G, Gerasimaitė R. The use of hoechst dyes for DNA staining and beyond[J]. Chemosensors, 2018, 6(2): 18.
[3] Thai N Q, Tseng N H, Vu M T, et al. Discovery of DNA dyes Hoechst 34580 and 33342 as good candidates for inhibiting amyloid beta formation: in silico and in vitro study[J]. Journal of Computer-Aided Molecular Design, 2016, 30(8): 639-650.