HBC (HBC 530) is a GFP-like synthetic dye with the maximum excitation wavelength of 485nm, and the maximum emission wavelength of 530nm [1]. HBC features a structurally rigid electron acceptor and a strong electron donor, which can bind to RNA of different lengths and emit intense fluorescence[2]. The unique fluorescence imaging channel provided by HBC has been widely used in the engineering of luminometric sensor platforms for multiple colors RNA labeling or intracellular RNA-RNA interaction imaging [3]. HBC can be used for in situ localization of tumor-specific proteases and animal optical in in vitro imaging analysis experiments [4].
References:
[1] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] Bereiter R, Micura R. Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA[J]. Beilstein Journal of Organic Chemistry, 2025, 21(1): 727-735.
[3] Tang H, Peng J, Peng S, et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. Chemical Science, 2022, 13(47): 14032-14040.
[4] Xie X, Sun Y, Peng J, et al. Collagen anchoring protein-nucleic acid chimeric probe for in situ in vivo mapping of a tumor-specific protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
HBC (HBC 530)是一种类GFP的合成染料，最大激发波长为485nm，最大发射波长为530nm[1]。HBC具有结构刚性的电子受体和强电子给体，能与不同长度的RNA结合并发出强烈的荧光[2]。HBC提供的独特荧光成像通道已广泛用于发光传感器平台的工程化，以实现多色RNA标记或细胞内RNA-RNA相互作用成像[3]。HBC可用于肿瘤特异性蛋白酶的原位定位以及体外光学成像分析实验[3]。