H-L-Photo-lysine hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. H-L-Photo-lysine hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. H-L-Photo-lysine hydrochloride acts as a UV light-activated photo-crosslinking probe[1][2][3].
Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications[2].
[1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7. [2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. [3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.