Gsafe Red Plus Nucleic Acid Dye (10000X) is a red fluorescent nucleic acid stain that has been optimized and developed on the basis of Gelred with gel staining characteristics and is designed to replace the highly toxic staining agent ethidium bromide (EB) with higher clarity and sensitivity. Because Gsafe Red Plus has the same spectral characteristics as EB, it can be used to replace EB without changing any imaging system. If you use SYBR (such as SYBR Green I / SYBR Gold) stain and use a UV transilluminator to observe the gel, you can use Gsafe Red Plus to replace the SYBR stain, without replacing the existing SYBR light. sheet. However, Plus cannot be sufficiently excited under a 488 nm laser or similar visible light. If necessary, it is recommended that you use Gsafe Green stain. Its sensitivity is the same as SYBR Green I, but its stability and reliability are far better than the latter. Plus can be used for both precast gel staining and post gel staining. Generally, post-staining can obtain more sensitive characteristics than pre-staining, and can exclude the possibility that the staining agent will have any effect on the separation of nucleic acid bands during the electrophoresis process. However, front dyeing is simpler and more economical than back dyeing because front dyeing does not require an additional coloring process and the amount of dye used is less. In addition, like GelGreen and EvaGreen, Plus has extremely low ability to induce mutations compared to EB or SYBR. Gsafe Red Plus nucleic acid dye, 10,000X in water is concentrated Gsafe Red Plus solution. When used for pre-dyeing, it can be diluted 10,000 times before use; when used for post-dyeing, it is recommended that you dilute it 3,300 times before use, see specific steps.
Gsafe Red Plus核酸染料（10000倍浓度）是一种红色荧光核酸染料，基于Gelred进行了优化和开发，具有凝胶染色特性，并旨在用更高的清晰度和灵敏度替代高毒性的染色剂溴化乙锭（EB）。由于Gsafe Red Plus具有与EB相同的光谱特性，因此可以在不改变任何成像系统的情况下替换EB。如果您使用SYBR（例如SYBR Green I / SYBR Gold）染料并使用紫外线透射仪观察凝胶，则可以使用Gsafe Red Plus替换SYBR染料，而无需更换现有的SYBR照明板。但是，在488 nm激光或类似可见光下不能足够地激发Plus。如有必要，请使用Gsafe Green染料。它的灵敏度与SYBR Green I相同，但其稳定性和可靠性远远优于后者。Plus可用于预制凝胶染色和后处理凝胶染色。通常情况下，后着色比前着色获得更敏感的特征，并且可以排除着色剂对电泳过程中核酸带分离产生任何影响的可能性。然而，前染色比后染色更简单、更经济，因为前染色不需要额外的着色过程，并且使用的染料量较少。此外，与EB或SYBR相比，像GelGreen和EvaGreen一样，Plus诱导突变的能力极低。 Gsafe Red Plus核酸染料在水中10,000倍浓缩是Gsafe Red Plus溶液。当用于预着色时，在使用之前可以将其稀释10,000倍；当用于后着色时，建议您在使用之前将其稀释3,300倍，请参阅具体步骤。