GPDA (GPN) is the substrate for detecting glycylproline dipeptidyl aminopeptidase. GPDA (GPN) can be hydrolyzed by the corresponding enzyme, producing yellow p-nitroaniline. By monitoring the change in absorbance at 405nm, enzyme activity can be quantified[1].
References:
[1] Hino M, Fuyamada H, Hayakawa T, et al. X-Prolyl dipeptidyl-aminopeptidase activity, with X-proline p-nitroanilides as substrates, in normal and pathological human sera. Clin Chem. 1976 Aug;22(8):1256-61.
GPDA(GPN)是甘氨酰脯氨酸二肽氨基肽酶（Glycylproline Dipeptidyl Aminopeptidase）检测的底物。GPDA（GPN）可作为底物被相应的酶水解，生成黄色的对硝基苯胺，通过监测405nm波长下的吸光度变化，即可定量酶活性[1]。