GnRH II (trifluoroacetate salt)

Gonadotropin-releasing hormone II (GnRH II) is a peptide agonist of the GnRH receptor (GnRHR). It binds to human recombinant type I and marmoset type II GnRHRs (IC50s = 26.1 and 1.07 nM, respectively, in COS-7 cells) and stimulates inositol phosphate production (EC50s = 7.41 and 0.45 nM, respectively). GnRH II stimulates luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release from cultured primary rhesus monkey anterior pituitary cells (EC50s = 0.37 and 0.59 nM, respectively) and dose-dependently stimulates LH and FSH release from cultured primary rat anterior pituitary cells. In vivo, GnRH II (1 μg/kg, i.v.) increases plasma LH levels in White Leghorn pullets but does not affect plasma FSH levels. It also decreases food intake in zebrafish and increases courtship behavior in female white-crowned sparrows when administered intracerebroventricularly at doses of 1 pmol/g and 100 ng, respectively.