Glyco-diosgenin is a synthetic surfactant and detergent, which is used to extract proteins from membranes for structural and functional studies[1]. Glyco-diosgenin is widely used to stabilize various membrane proteins in solutions outside the lipid bilayer[2]. Glyco-diosgenin can be used for purifying the Escherichia coli TatBC complex and facilitating the binding of the complex to the precursor protein[3].
References:
[1] van Dalsen L, Weichert D, Caffrey M. In meso crystallogenesis. Compatibility of the lipid cubic phase with the synthetic digitonin analogue, glyco-diosgenin[J]. Applied Crystallography, 2020, 53(2): 530-535.
[2] Ehsan M, Wang H, Katsube S, et al. Glyco‐Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study[J]. ChemBioChem, 2022, 23(7): e202200027.
[3] Wojnowska M, Gault J, Yong S C, et al. Precursor–Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation[J]. Biochemistry, 2018, 57(10): 1663-1671.
Glyco-diosgenin是一种合成型表面活性剂和去垢剂，用于从膜中提取蛋白质以进行结构和功能研究[1]。Glyco-diosgenin被广泛用于在脂双层外的溶液中稳定各种膜蛋白[2]。Glyco-diosgenin可用于纯化大肠杆菌TatBC复合物，并促进该复合物与前体蛋白的结合[3]。