PAR3 (1-6) amide is a synthetic peptide agonist of proteinase-activated receptor 1 (PAR1) and PAR2 that corresponds to residues 1-6 of the amino terminal tethered ligand sequence of human PAR3 and residues 39-44 of the full-length human sequence. PAR3 (1-6) amide activates PAR1 and PAR2 in Jurkat and HEK cells, inducing calcium accumulation in Jurkat cells, which is reduced following desensitization with the PAR1- and PAR2-selective peptide agonist SFLLR-NH2.1 It decreases the expression of CDC42 in wild-type, but not PAR3 knockdown, PANC-1 cells and reduces invasion of PANC-1 cells.2
References
1. Hansen, K.K., Saifeddine, M., and Hollenberg, M.D. Tethered ligand-derived peptides of proteinase-activated receptor 3 (PAR3) activate PAR1 and PAR2 in Jurkat T cells. Immunology 112(2), 183-190 (2004).
2. Segal, L., Katz, L.S., Lupu-Meiri, M., et al. Proteinase-activated receptors differentially modulate in vitro invasion of human pancreatic adenocarcinoma PANC-1 cells in correlation with changes in the expression of CDC42 protein. Pancreas 43(1), 103-108 (2014).