N6-Methyladenosine 5'-monophosphate (sodium salt) is an activator of glycogen phosphorylase b (Ka=22µM)[1] and a non-competitive inhibitor of rat adenylate kinase II (Ki=4.2mM)[2]. N6-Methyladenosine 5'-monophosphate (sodium salt) is the 5'-monophosphate form of N6-methyladenosine (m6A), usually in the form of a sodium salt. N6-methyladenosine (m6A) is a methylated adenosine molecule in which the N6 position of adenine is methylated. m6A, a common form of RNA modification, mainly exists in mRNA and affects its stability, translation efficiency and gene expression regulation[3]. N6-Methyladenosine 5'-monophosphate (sodium salt) is often used as a research tool to investigate the mechanism of action of m6A modification in different biological processes. N6-Methyladenosine 5'-monophosphate (sodium salt) is primarily used in research on RNA epigenetics, particularly for studying the mechanisms of m6A modification[4][5], RNA-protein interactions[6], and its role in gene expression regulation[7].
References:
[1] Groner, Y., Grosfeld, H., & Littauer, U. Z. (1976). 5'-Capping structures of Artemia salina mRNA and the translational inhibition by cap analogs. European journal of biochemistry, 71(1), 281–293.
[2] Hai, T. T., Abo, M., & Hampton, A. (1982). Species- or isozyme-specific enzyme inhibitors. 9. Selective effects in inhibitions of rat pyruvate kinase isozymes by adenosine 5'-diphosphate derivatives. Journal of medicinal chemistry, 25(10), 1184–1188.
[3] Liu, Y., Yang, D., Liu, T., Chen, J., Yu, J., & Yi, P. (2023). N6-methyladenosine-mediated gene regulation and therapeutic implications. Trends in molecular medicine, 29(6), 454–467.
[4] Chen, Y., Wu, Y., Zhu, L., Chen, C., Xu, S., Tang, D., Jiao, Y., & Yu, W. (2022). METTL3-Mediated N6-Methyladenosine Modification of Trim59 mRNA Protects Against Sepsis-Induced Acute Respiratory Distress Syndrome. Frontiers in immunology, 13, 897487.
[5] Yu, J., Li, Y., Wang, T., & Zhong, X. (2018). Modification of N6-methyladenosine RNA methylation on heat shock protein expression. PloS one, 13(6), e0198604.
[6] Fry, N. J., Law, B. A., Ilkayeva, O. R., Holley, C. L., & Mansfield, K. D. (2017). N6-methyladenosine is required for the hypoxic stabilization of specific mRNAs. RNA (New York, N.Y.), 23(9), 1444–1455.
[7] Nagarajan, A., Janostiak, R., & Wajapeyee, N. (2019). Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA. Methods in molecular biology (Clifton, N.J.), 1870, 263–271.
N6-Methyladenosine 5'-monophosphate (sodium salt)是糖原磷酸化酶b的激活剂(Ka=22µM)[1] 和大鼠腺苷酸激酶II 的非竞争性抑制剂(Ki=4.2mM)[2]。N6-Methyladenosine 5'-monophosphate (sodium salt) 是N6-甲基腺苷（m6A）的5'-单磷酸形式，通常以钠盐的形式存在。N6-甲基腺苷（m6A）是一种甲基化的腺苷分子，其中腺嘌呤的N6位置被甲基化。m6A是一种常见的RNA修饰形式，主要存在于mRNA中，影响mRNA的稳定性、翻译效率和基因表达调控[3]。N6-Methyladenosine 5'-monophosphate (sodium salt) 常被用作研究m6A修饰在不同生物过程中的作用机制的研究工具。N6-Methyladenosine 5'-monophosphate (sodium salt) 主要用于研究RNA表观遗传学，特别是m6A修饰机制[4][5]、RNA-蛋白质相互作用[6]及其在基因表达调控中的作用[7]。