Diolein is a type of diacylglycerol (DAG), composed of glycerol and two oleic acid molecules linked by ester bonds. DAG could function as a second messengers in various cell signaling pathways, particularly in activating protein kinase C (PKC)[1][2]. Diolein is widely used in cell biology research to study lipid signaling[3], enzyme activity[4], and membrane dynamics[5], and is a very promising non-viral vector for in vivo gene delivery[6].
References:
[1]. McPhail LC, Clayton CC, Snyderman R. A potential second messenger role for arachidonic acid: activation of Ca2+-dependent protein kinase. Trans Assoc Am Physicians. 1984;97:222-31. PMID: 6242084.
[2]. Mosior M, Epand RM. Mechanism of activation of protein kinase C: roles of diolein and phosphatidylserine. Biochemistry. 1993 Jan 12;32(1):66-75. doi: 10.1021/bi00052a010. PMID: 8418861.
[3]. Oishi K, Raynor RL, Charp PA, Kuo JF. Regulation of protein kinase C by lysophospholipids. Potential role in signal transduction. J Biol Chem. 1988 May 15;263(14):6865-71. PMID: 3360811.
[4]. Duan ZQ, Du W, Liu DH. The mechanism of solvent effect on the positional selectivity of Candida antarctica lipase B during 1,3-diolein synthesis by esterification. Bioresour Technol. 2011 Dec;102(23):11048-50. doi: 10.1016/j.biortech.2011.09.003. Epub 2011 Sep 18. PMID: 21978621.
[5]. van Gorkom LC, Nie SQ, Epand RM. Hydrophobic lipid additives affect membrane stability and phase behavior of N-monomethyldioleoylphosphatidylethanolamine. Biochemistry. 1992 Jan 28;31(3):671-7. doi: 10.1021/bi00118a006. PMID: 1731922.
[6]. Guo W, Gosselin MA, Lee RJ. Characterization of a novel diolein-based LPDII vector for gene delivery. J Control Release. 2002 Sep 18;83(1):121-32. doi: 10.1016/s0168-3659(02)00167-0. PMID: 12220844.
Diolein 是一种二酰基甘油 (DAG)，由甘油和两个油酸分子通过酯键连接而成。DAG 可以在各种细胞信号通路中充当次级信使，特别是在激活蛋白激酶 C (PKC) 中[1][2]。Diolein 广泛用于细胞生物学研究，以研究脂质信号传导[3]、酶活性[4]和膜动力学[5]，并且是一个很有希望的用于体内基因递送的非病毒载体[6]。