Ethyl glucuronide是乙醇的一种非挥发性、水溶性的直接代谢物，可在体液、组织和毛发中检测到。
Cas No.:17685-04-0
Sample solution is provided at 25 µL, 10mM.
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Ethyl glucuronide is a nonvolatile, water-soluble, direct metabolite of ethanol, which can be detected in body fluids, tissues, and hair [1]. Ethyl glucuronide content is positively correlated with alcohol intake and can be used as a marker for the detection of alcohol abuse[2]. Ethyl glucuronide can be used as a standard internal control to develop a liquid chromatography-tandem mass spectrometry method for the identification of related compounds[3].
In vitro, Ethyl glucuronide treatment (100μM) for 24h blocked the formation of TLR4-MD2 complex and stimulated the expression of TLR4-dependent SEAP protein in HEK-TLR4 cells[4].
In vivo, Ethyl glucuronide treatment via intrathecal injection at a single dose of 0.38µg caused significant hyperalgesia in rats within 3 hours[4].
References:
[1] Wurst F M, Kempter C, Metzger J, et al. Ethyl glucuronide: a marker of recent alcohol consumption with clinical and forensic implications[J]. Alcohol, 2000, 20(2): 111-116.
[2] Helander A, Böttcher M, Fehr C, et al. Detection times for urinary ethyl glucuronide and ethyl sulfate in heavy drinkers during alcohol detoxification[J]. Alcohol & Alcoholism, 2009, 44(1): 55-61.
[3] Wurst F M, Kempter C, Seidl S, et al. Ethyl glucuronide--a marker of alcohol consumption and a relapse marker with clinical and forensic implications[J]. Alcohol and alcoholism (Oxford, Oxfordshire), 1999, 34(1): 71-77.
[4] Lewis S S, Hutchinson M R, Zhang Y, et al. Glucuronic acid and the ethanol metabolite ethyl-glucuronide cause toll-like receptor 4 activation and enhanced pain[J]. Brain, behavior, and immunity, 2013, 30: 24-32.
Ethyl glucuronide是乙醇的一种非挥发性、水溶性的直接代谢物，可在体液、组织和毛发中检测到[1]。Ethyl glucuronide的含量与酒精摄入量呈正相关，可作为检测酗酒的标志物[2]。Ethyl glucuronide可用作标准内参，以开发用于鉴定相关化合物的液相色谱-串联质谱法[3]。
在体外，使用100µM的Ethyl glucuronide处理24小时，阻断了HEK-TLR4细胞中TLR4-MD2复合物的形成，并刺激了TLR4依赖性SEAP蛋白的表达[4]。
在体内，单次鞘内注射0.38µg剂量的Ethyl glucuronide，在3小时内导致大鼠出现显著的痛觉过敏[4]。
| Cell experiment [1]: | |
Cell lines | HEK-TLR4 cells |
Preparation Method | HEK-TLR4 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) and antibiotics (penicillin 1000U/ml, streptomycin 10mg/ml) under normal conditions (5% CO2 with 95% humidified air). HEK-TLR4 cells (5×103 per well) were inoculated in a 96-well cell culture plate and incubated at 37°C with 5% CO2 for 48h. Then, the cells were treated with different concentrations of Ethyl glucuronide (0.01, 0.1, 1, 10, 100µM) for 48h, respectively, and the supernatant (15μl) was then collected from each well for measurement of SEAP levels. |
Reaction Conditions | 0.01, 0.1, 1, 10, 100µM; 24h |
Applications | Ethyl glucuronide treatment enhanced the levels of SEAP within HEK-TLR4 cells in a dose-dependent manner. |
References: | |
| Cas No. | 17685-04-0 | SDF | |
| 别名 | 乙基Β-D-葡萄糖醛酸 | ||
| Canonical SMILES | OC([C@@H]1-;@[C@H](-;@[C@@H](-;@[C@@H](O)-;@[C@H](OCC)-;@O-;@1)O)O)=O | ||
| 分子式 | C8H14O7 | 分子量 | 222.19 |
| 溶解度 | H2O : 125 mg/mL (562.58 mM; Need ultrasonic) | 储存条件 | Store at -20°C, protect from light |
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5007 mL | 22.5033 mL | 45.0065 mL |
| 5 mM | 900.1 μL | 4.5007 mL | 9.0013 mL |
| 10 mM | 450.1 μL | 2.2503 mL | 4.5007 mL |
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动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
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2.
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