Coumarin 6 is a stable laser dye (λex=450nm, λem=500nm) and is a useful probe in variety of chemical and physicochemical studies[1]. Coumarin 6 is used as a fluorescent dye for microplastics, is soluble in organic solvents but insoluble in water, Coumarin 6 emits intense green fluorescence when exposed to blue light[2]. Due to the extremely low solubility (with a solubility in water lower than 0.25μg/ml), Coumarin 6 can be regarded as a typical hydrophobic model drug[3]. Coumarin 6 can be encapsulated in single-chain liposomes and administered by eye drops, which is used for retinal flat imaging in animal models[4]. Coumarin 6 can form a complex with β-cyclodextrin, which can be used to quickly distinguish different types of cells[5].
References:
[1] Satpati A K, Kumbhakar M, Maity D K, et al. Photophysical investigations of the solvent polarity effect on the properties of coumarin-6 dye[J]. Chemical physics letters, 2005, 407(1-3): 114-118.
[2] Cheng Y, Zhang J, Nakano H, et al. Coumarin 6 staining method to detect microplastics[J]. Marine Pollution Bulletin, 2023, 193: 115167.
[3] Miao X, Li Y, Wang X, et al. Transport mechanism of coumarin 6 nanocrystals with two particle sizes in MDCKII monolayer and larval zebrafish[J]. ACS Applied Materials & Interfaces, 2016, 8(20): 12620-12630.
[4] Inokuchi Y, Hironaka K, Fujisawa T, et al. Physicochemical properties affecting retinal drug/coumarin-6 delivery from nanocarrier systems via eyedrop administration[J]. Investigative ophthalmology & visual science, 2010, 51(6): 3162-3170.
[5] Edetsberger M, Knapp M, Gaubitzer E, et al. Effective staining of tumor cells by coumarin-6 depends on the stoichiometry of cyclodextrin complex formation[J]. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2011, 70(3): 327-331.
Coumarin 6是一种稳定的激光染料（λex=450nm, λem=500nm），是多种化学和物理化学研究中的有用探针[1]。Coumarin 6被用作微塑料的荧光标记物，可溶于有机溶剂但不溶于水，在蓝光照射下会发出强烈的绿色荧光[2]。由于极低的水溶性（低于0.25μg/ml），Coumarin 6可被视为典型的疏水性模型药物[3]。Coumarin 6可被包裹在单链脂质体中并通过滴眼剂给药，用于动物模型的视网膜平面成像[4]。Coumarin 6能与β-环糊精形成复合物，可用于快速区分不同类型的细胞[5]。