Substance P 1-7

Animal experiment:

Rats[1]Sprague-Dawley male rats weighing 250-300 g are anaesthetized with halothane and placed in a stereotaxic frame. An injection cannula, conically shaped with a penetration tip diameter of approximately 0.15 mm is loared into the SNR. Saline (0.2 μL), SP (0.007-0.7 nM) or Substance P (7-11) (0.01-1 nM) is injected into the left substantia nigra, pars reticulata (SNR) and the rat is placed in a rotometer. The substances are injected in a total volume of 0.2 μL over a period of 1 min. A group of animals is sacrificed by decapitation 1 hour after the injection, their brains are immediately removed and tissue samples are taken from left and right striatum, globus pallidum (GP) and substantia nigra (SN). Samples are assayed for SP and SP(1-7)[1].Mice[2] The accumulated response time (s) of reciprocal movements of hindlimb scratching, biting, fore- and hindpaw licking are measured in Male mice (STD strain, 23-28 g) during the whole period of aversive response and 20 min at maximum. Substance P (7-11) is tested for its ability to inhibit the aversive response produced by intrathecal injection of SP or SP(5-11) (0.1 nM/mouse). Substance P (7-11) (1, 2, 4 pM) is then administered together with SP or SP(5-11)[2].

References:

[1]. Herrera-Marschitz M, et al. The substance P(1-7) fragment is a potent modulator of substance P actions in the brain. Brain Res. 1990 Jun 25;521(1-2):316-20.
[2]. Sakurada T, et al. Substance P(1-7) antagonizes substance P-induced aversive behaviour in mice. Neurosci Lett. 1988 Dec 19;95(1-3):281-5.