| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 5mg | ¥2,070.00 | 现货 | 1 | |
| 10mg | ¥3,555.00 | 现货 | 1 | |
| 50mg | ¥0.01 | 现货 | 1 | |
| 100mg | ¥0.01 | 现货 | 1 |
N-Nornuciferine is an alkaloid that has been found in A. grandifolia and has cholinesterase inhibitory activity.1 It is an inhibitor of butyrylcholinesterase (BChE; IC50 = 5.6 ?M for the mouse enzyme).
1.Cometa, M.F., Fortuna, S., Palazzino, G., et al.New cholinesterase inhibiting bisbenzylisoquinoline alkaloids from Abuta grandifoliaFitoterapia83(3)476-480(2012)
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Kinase experiment: |
For the determination of the inhibition constant Ki values, various final concentrations of the specific substrate dextromethorphan for CYP2D6 in the range of 1 to 10 mM and different concentrations of the N-Nornuciferine in the range of 0 to 25 mM are used. After preincubation for 10 min, the reactions are initiated by the addition of NADPH. Each incubation test is performed in triplicate. Thirty minutes after the incubation is initiated, the reaction is stopped by the addition of 100 mL of ice-cold acetonitrile containing 1 mg/mL propranolol (IS). The incubation mixtures are then centrifuged at 15,000g for 10 min at 4 °C. Ten-microliter aliquots of the supernatants are injected into an LC-MS/MS system[1]. |
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References: [1]. Ye LH, et al. Identification and characterization of potent CYP2D6 inhibitors in lotus leaves. J Ethnopharmacol. 2014 Apr 11;153(1):190-6. |
