Neotuberostemonine is an alkaloid originally isolated from S. tuberosa that has diverse biological activities.1,2,3 It inhibits LPS-induced increases in inducible nitric oxide synthase (iNOS) protein levels and NO production in RAW 264.7 cells when used at a concentration of 100 ?M.1 Neotuberostemonine (50 ?M) inhibits RANKL-induced osteoclast differentiation of RAW 264.7 cells.2 It reduces the citric acid-induced cough reflex in conscious guinea pigs when administered at a dose of 133 ?mol/kg.3 Neotuberostemonine (40 mg/kg) inhibits pulmonary collagen deposition and fibrosis, as well as bronchoalveolar lavage fluid (BALF) monocyte and lymphocyte infiltration in a mouse model of bleomycin-induced pulmonary fibrosis.1
1.Xiang, J., Cheng, S., Feng, T., et al.Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophagesInt. Immunopharmacol.36158-164(2016) 2.Yun, J., Lee, K.Y., and Park, B.Neotuberostemonine inhibits osteoclastogenesis via blockade of NF-kB pathwayBiochimie15781-91(2019) 3.Chung, H.-S., Hon, P.-M., Lin, G., et al.Antitussive activity of Stemona alkaloids from Stemona tuberosaPlanta Med.69(10)914-920(2003)