Neoandrographolide is a diterpenoid compound isolated from Andrographis paniculata, which exhibits anti‑inflammatory and lipid‑lowering effects[1-2]. Neoandrographolide is applicable for research on antiviral, antitumor, cardiovascular and cerebrovascular diseases, osteoporosis, and type 2 diabetes[3-4].
In vitro, co‑treatment of RAW 264.7 macrophages with Neoandrographolide (30–150μM) and LPS (5μg/ml) for 24–36h. Neoandrographolide significantly inhibited the production of TNF‑α and nitric oxide (NO)[5]. pretreatment of bone marrow‑derived mouse macrophages with Neoandrographolide (1.5–90μM) for 2h, followed by stimulation with LPS (5μg/ml) for 24h. Neoandrographolide markedly suppressed the expression of iNOS and COX‑2, and simultaneously reduced the generation of NO and PGE2[6].
In vivo, pretreatment of C57BL/6 mice with Neoandrographolide (10mg/kg/day) via intraperitoneal injection for 6 days prior to establishing a myocardial ischemia/reperfusion injury model. Neoandrographolide significantly improved cardiac systolic function, reduced myocardial infarct area and inflammatory cell infiltration, and inhibited cardiomyocyte apoptosis[7]. In an ovariectomy‑induced osteoporosis model, C57BL/6J mice were treated with Neoandrographolide (15mg/kg/day and 30mg/kg/day) by intraperitoneal injection for 8 weeks. Neoandrographolide significantly alleviated bone loss and improved trabecular microstructural parameters[8].
References:
[1] Ghosh N, Ghosh R, Mandal V, et al. Recent advances in herbal medicine for treatment of liver diseases. Pharm Biol. 2011 Sep;49(9):970-88.
[2] Yang T, Shi HX, Wang ZT, et al. Hypolipidemic effects of andrographolide and neoandrographolide in mice and rats. Phytother Res. 2013 Apr;27(4):618-23.
[3] Sangeetha K, Martín-Acebes MA, Saiz JC, et al. Molecular docking and antiviral activities of plant derived compounds against zika virus. Microb Pathog. 2020 Dec;149:104540.
[4] Jadhav AK, Karuppayil SM. Andrographis paniculata (Burm. F) Wall ex Nees: Antiviral properties. Phytother Res. 2021 Oct;35(10):5365-5373.
[5] Liu J, Wang ZT, Ji LL. In vivo and in vitro anti-inflammatory activities of neoandrographolide. Am J Chin Med. 2007;35(2):317-28.
[6] Liu J, Wang ZT, Ji LL, et al. Inhibitory effects of neoandrographolide on nitric oxide and prostaglandin E2 production in LPS-stimulated murine macrophage. Mol Cell Biochem. 2007 Apr;298(1-2):49-57.
[7] Liu Y, Liu Y, Zhang HL, et al. Amelioratory effect of neoandrographolide on myocardial ischemic-reperfusion injury by its anti-inflammatory and anti-apoptotic activities. Environ Toxicol. 2021 Dec;36(12):2367-2379.
[8] Tang K, Deng W, Huang Z, et al. Neoandrographolide inhibits mature osteoclast differentiation to alleviate bone loss and treat osteoporosis. Front Pharmacol. 2025 Feb 11;16:1466057.
Neoandrographolide是一种从穿心莲中分离的二萜类化合物，具有抗炎和降脂的作用[1-2]。Neoandrographolide可用于抗病毒、抗肿瘤、心脑血管疾病、骨质疏松症和2型糖尿病等相关研究[3-4]。
在体外，Neoandrographolide（30–150μM）与LPS（5μg/ml）共同处理RAW 264.7巨噬细胞24–36小时，显著抑制TNF-α和一氧化氮（NO）的产生[5]。Neoandrographolide（1.5–90μM）预处理骨髓来源的小鼠巨噬细胞2小时，随后以LPS（5μg/ml）刺激24小时，显著抑制iNOS和COX-2的表达，同时降低NO和PGE2的产生[6]。
在体内，Neoandrographolide（10mg/kg/day）通过腹腔注射预处理C57BL/6小鼠6天，随后建立心肌缺血/再灌注损伤模型。Neoandrographolide显著改善了心脏收缩功能、减少了心肌梗死面积和炎症细胞浸润，同时抑制了心肌细胞凋亡[7]。Neoandrographolide（15mg/kg/day和30mg/kg/day）通过腹腔注射给药，用于处理经双侧卵巢切除术建立的C57BL/6J小鼠骨质疏松模型8周。Neoandrographolide显著减轻了骨量丢失，并改善了骨小梁微观结构参数[8]。