Hoechst 34580 tetrahydrochloride is a Hoechst series dye that is commonly used for nuclear labeling and staining of living cells. The maximum excitation/emission light is 392⁄490nm respectively. Hoechst dyes can be used to monitor cell viability by tracking changes in their emission spectra. As slightly groove-binding DNA stains with AT selectivity, Hoechst dyes are able to bind to all nucleic acids, but they show greater fluorescence enhancement for AT-rich double-stranded DNA strands compared to GC-rich strands[1]. This property has been used to identify Q bands in chromosomes, which are AT base pair-rich regions that fluoresce brightly when stained with quinacrine dye[2]. The fluorescence intensity of Hoechst dye increases as the pH of the solution increases.
Hoechst 34580 tetrahydrochloride is commonly used as a cell-permeable nuclear counterstain that emits blue fluorescence upon binding to dsDNA. Hoechst 34580 tetrahydrochloride is also used in various studies related to cell counting, cell cycle analysis and cell replication. It is particularly useful for identifying condensed nuclei in apoptotic cells and for cell cycle studies in combination with BrdU staining.
Hoechst 34580 tetrahydrochloride是Hoechst系列染料，常用于活细胞的细胞核标记染色，最大激发/发射光分别为392⁄490nm。Hoechst染料可被用于通过跟踪其发射光谱的变化来监测细胞活力。作为具有AT选择性的轻微凹槽结合DNA染色剂，Hoechst染料能够与所有核酸结合，但与富含GC的链相比，它们对富含AT的双链DNA链显示出更大的荧光增强[1]。该特性已被用于鉴定染色体中的Q条带，这些染色体是富含AT碱基对的区域，当用奎纳克林染料染色时会发出明亮的荧光[2]。Hoechst染料的荧光强度随着溶液的pH的增加而增加。
Hoechst 34580 tetrahydrochloride常用作细胞可渗透的核复染剂，与dsDNA结合后发出蓝色荧光。Hoechst 34580 tetrahydrochloride也被用于与细胞计数、细胞周期分析和细胞复制相关的各类研究。它对于识别凋亡细胞中的凝聚核以及与 BrdU 染色结合进行细胞周期研究特别有用。
References:
[1].Karen E. Selph. Enumeration of marine microbial organisms by flow cytometry using near-UV excitation of Hoechst 34580-stained DNA.
[2]. Jonas Bucevičius,Gražvydas Lukinavičius , Rūta Gerasimaitė. The Use of Hoechst Dyes for DNA Staining and Beyond. Chemosensors 2018, 6(2), 18. Doi:org/10.3390/chemosensors6020018.