β-Glucuronidase from bovine liver is an important lysosomal enzyme involved in the degradation of glucuronic acid-containing glycosaminoglycans[1]. β-Glucuronidase catalyzes the hydrolysis of glucuronic acid derivatives and plays a key physiological role in humans, animals, plants and some bacteria[2]. β-Glucuronidase has been measured as a typical lysosomal enzyme during subcellular fractionation studies[3]. β-Glucuronidase has become a model system for studying genetic regulatory polymorphisms, androgen induction mechanisms, lysosomal enzyme processing and intracellular enzyme localization mechanisms[4]. β-Glucuronidase deficiency causes mucopolysaccharidosis type VII (MPSVII), leading to lysosomal accumulation in the brain[5]. β-Glucuronidase has a dissociative effect on bilirubin and can convert conjugated bilirubin into an unconjugated form[6].
Activity: ≥100,000units/g.
Definition of enzyme activity: At 37°C and pH 5.0, a modified Fishman apparatus will release 1.0μg of phenolphthalein from phenolphthalein glucuronide per hour.
References:
[1] Brüsselbach S. Extracellular β-Glucuronidase for Gene-Directed Enzyme-Prodrug Therapy[J]. Suicide Gene Therapy: Methods and Reviews, 2004: 303-330.
[2] Ibrahim N E D, Shao W, Ma K. Properties and Biotechnological Applications of β-Glucuronidases[M]//Biotechnology of Microorganisms. Apple Academic Press, 2019: 147-178.
[3] Fishman W H. β-Glucuronidase[M]//Methods of enzymatic analysis. Academic Press, 1974: 929-943.
[4] Paigen K. Mammalian β-glucuronidase: genetics, molecular biology, and cell biology[J]. Progress in nucleic acid research and molecular biology, 1989, 37: 155-205.
[5] Roy E. Cell disorders in lysosomal storage diseases[D]. Université René Descartes-Paris V, 2012.
[6] Chowdhury N R, Li Y, Chowdhury J R. Disorders of bilirubin metabolism[J]. The liver: biology and pathobiology, 2020: 229-244.
β-Glucuronidase from bovine liver是一种重要的溶酶体酶，参与含葡萄糖醛酸的糖胺聚糖的降解[1]。β-Glucuronidase能够催化葡萄糖醛酸衍生物的水解，该酶在人类、动物、植物和一些细菌中发挥关键生理作用[2]。在亚细胞组分化研究期间，β-Glucuronidase已被作为典型的溶酶体酶测量[3]。β-Glucuronidase已成为研究遗传调控多态性、雄激素诱导机制、溶酶体酶加工和细胞内酶定位机制的模型系统[4]。β-Glucuronidase缺乏会引起粘多糖沉积症VII型（MPSVII），导致大脑中的溶酶体蓄积[5]。β-Glucuronidase对胆红素具有解离作用，能够将结合胆红素转化为非结合形式[6]。
酶活性：≥100,000units/g。
酶活性定义：在37℃、pH 5.0条件下，一个改良Fishman装置每小时将从酚酞葡糖苷酸中释放1.0μg酚酞。